ArticleMolecules (Basel, Switzerland)2021
Validation of an Analytical Method for the Simultaneous Determination of Hyaluronic Acid Concentration and Molecular Weight by Size-Exclusion Chromatography.
Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Use of Benzene-Free Carbomer 980 in the Formulation of Metronidazole Gel 0.75% and Demonstration of Equivalence with Carbomer 940.AAPS PharmSciTech · 2026Article
- Review
- Review
- Agarose Gel Electrophoresis Reveals the Molecular Weight Distribution of Hyaluronan Produced by Orbital Fibroblasts.Gels (Basel, Switzerland) · 2025Article
- Hyaluronic Acid Interactions with Pork Myofibrillar Proteins in Emulsion Gel-Type Systems.Molecules (Basel, Switzerland) · 2025Article
- Inverted Pyramid Nanostructures Coupled with a Sandwich Immunoassay for SERS Biomarker Detection.Nanomaterials (Basel, Switzerland) · 2025Article
- Evaluation of mango residues to produce hyaluronic acid by Streptococcus zooepidemicus.Folia microbiologica · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The hyaluronic acid (HA) global market growth can be attributed to its use in medical, cosmetic, and pharmaceutical applications; thus, it is important to have validated, analytical methods to ensure confidence and security of its use (and to save time and resources). In this work, a size-exclusion chromatography method (HPLC-SEC) was validated to determine the concentration and molecular distribution of HA simultaneously. Analytical curves were developed for concentration and molecular weight in the ranges of 100-1000 mg/L and 0.011-2.200 MDa, respectively. The HPLC-SEC method showed repeatability and reproducibility greater than 98% and limits of detection and quantification of 12 and 42 mg/L, respectively, and was successfully applied to the analysis of HA from a bacterial culture, as well as cosmetic, and pharmaceutical products.
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Registered trials
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