ArticleFrontiers in bioengineering and biotechnology2021
Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts.
Article in Frontiers in bioengineering and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Tardigrade-Derived Strategy for Low-Cost Storage of Cell-Free Expression Lysates.ACS synthetic biology · 2026Article
- International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equity.Science advances · 2026Article
- Synthetic toehold biosensors for detectingFrontiers in microbiology · 2026Article
- Recent Advances in Developing Cell-Free Protein Synthesis Biosensors for Medical Diagnostics and Environmental Monitoring.Biosensors · 2025Review
- Detection of TuMV by a toehold switch sensor coupled with NASBA amplification in Pseudostellaria heterophylla.Plant methods · 2025Article
- qByte: An open-source isothermal fluorimeter for democratizing analysis of nucleic acids, proteins and cells.PLoS biology · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Article
- Bacterial Metallostasis: Metal Sensing, Metalloproteome Remodeling, and Metal Trafficking.Chemical reviews · 2024Review
- Thermostable in vitro transcription-translation compatible with microfluidic droplets.Microbial cell factories · 2024Article
- Validation of Cell-Free Protein Synthesis Aboard the International Space Station.ACS synthetic biology · 2024Article
- Streamlining the Detection of Human Thyroid Receptor Ligand Interactions with XL1-Blue Cell-Free Protein Synthesis and Beta-Galactosidase Fusion Protein Biosensors.Life (Basel, Switzerland) · 2023Article
- T7Max transcription system.Journal of biological engineering · 2023Article
- A Low-Cost, Thermostable, Cell-Free Protein Synthesis Platform for On-Demand Production of Conjugate Vaccines.ACS synthetic biology · 2023Article
- Article
- Rapid and Finely-Tuned Expression for Deployable Sensing Applications.Advances in biochemical engineering/biotechnology · 2023Article
- Current landscape and future directions of synthetic biology in South America.Frontiers in bioengineering and biotechnology · 2023Review
- Expanding luciferase reporter systems for cell-free protein expression.Scientific reports · 2022Article
- Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors.Biosensors · 2022Review
- Constructing Cell-Free Expression Systems for Low-Cost Access.ACS synthetic biology · 2022Article
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Authors and funding
10 authors.
Funding
Abstract
Cell-free gene expression systems have emerged as a promising platform for field-deployed biosensing and diagnostics. When combined with programmable toehold switch-based RNA sensors, these systems can be used to detect arbitrary RNAs and freeze-dried for room temperature transport to the point-of-need. These sensors, however, have been mainly implemented using reconstituted PURE cell-free protein expression systems that are difficult to source in the Global South due to their high commercial cost and cold-chain shipping requirements. Based on preliminary demonstrations of toehold sensors working on lysates, we describe the fast prototyping of RNA toehold switch-based sensors that can be produced locally and reduce the cost of sensors by two orders of magnitude. We demonstrate that these in-house cell lysates provide sensor performance comparable to commercial PURE cell-free systems. We further optimize these lysates with a CRISPRi strategy to enhance the stability of linear DNAs by knocking-down genes responsible for linear DNA degradation. This enables the direct use of PCR products for fast screening of new designs. As a proof-of-concept, we develop novel toehold sensors for the plant pathogen Potato Virus Y (PVY), which dramatically reduces the yield of this important staple crop. The local implementation of low-cost cell-free toehold sensors could enable biosensing capacity at the regional level and lead to more decentralized models for global surveillance of infectious disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.