Evidence map›Paper›PMID 34495871›Full record

ArticleAging2021

Effect of the Notch1-mediated PI3K-Akt-mTOR pathway in human osteosarcoma.

Kexiang Zhang, Song Wu, Hongwei Wu, Li Liu, Jiahui Zhou

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Aging, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
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  8. [Notch1/Akt/Foxo1 Pathway Regulated by Kisspeptin Is Involved in Endometrial Decidualization in Patients With Recurrent Spontaneous Abortion].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Kexiang ZhangDepartment of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China.
Song WuDepartment of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China.
Hongwei WuDepartment of Orthopedics, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, Hunan Province, China.
Li LiuDepartment of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China.
Jiahui ZhouDepartment of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China.
Central South University · CNThird Xiangya Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeOsteosarcoma is one of the most common malignant bone tumours in early adolescence. The incidence rate of osteosarcoma has stagnated over the past 30 years, highlighting the need to develop novel therapies. In osteosarcoma cells, Notch1 expression is absent, and the Notch1 pathway is related to cancer cell proliferation, apoptosis and autophagy. Our study aimed to investigate the role of Notch1 in osteosarcoma development.

methodsWe measured NICD1 expression induced by doxycycline treatment at various concentrations. The viability of human osteosarcoma cells (MG-63) induced by doxycycline was measured. Flow cytometry and cell apoptosis analysis were conducted to measure the effect of Notch1 on the cell cycle of human osteosarcoma cells. We also used a GFP-LC3 plasmid to detect Notch1-induced autophagy in MG-63 cells. Western blotting was conducted to analyse expression of the PI3K/Akt/mTOR signalling pathway through Notch1 induction by doxycycline.

resultsIn this study, we demonstrated that Notch1 activation by doxycycline potently suppressed cell proliferation by inducing S phase arrest in osteosarcoma cells. Doxycycline-induced Notch1 activation also induced apoptosis and autophagy in osteosarcoma cells. Moreover, we found that Notch1 inhibited PI3K/Akt/mTOR signalling to induce apoptosis and autophagy.

conclusionIn summary, our results revealed that Notch1 activation by doxycycline induces S phase arrest, apoptosis and autophagy by blocking PI3K/Akt/mTOR signalling in human osteosarcoma cells. Notch1 may be a potential clinical antitumour target for osteosarcoma therapy.

Indexed as

Anti-Bacterial AgentsBone NeoplasmsCell Cycle CheckpointsCell Line, TumorDoxycyclineGene Expression Regulation, NeoplasticHumansOsteosarcomaPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktReceptor, Notch1Signal TransductionS PhaseTOR Serine-Threonine KinasesUp-RegulationAnti-Bacterial AgentsDoxycyclineMTOR protein, humanNOTCH1 protein, humanPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktReceptor, Notch1TOR Serine-Threonine KinasesautophagyNotch1osteosarcomaPI3K/Akt/mTOR

Identifiers

PMID34495871
PMCPMC8457590
OpenAlexW3198132170

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.