Evidence map›Paper›PMID 34495521›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115(DE3) for Nontransgenic RNAi-Based Insect Pest Management.

Mabel L Taracena, Isabella Garcia Caffaro, Gabriela O Paiva-Silva, Pedro L Oliveira, Pedro A Rendon, Ellen M Dotson, Pamela M Pennington

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 60% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 5 countries.

Mabel L TaracenaDivision of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention (CDC), Center for Global Health, Atlanta, GA, USA. mabel.taracena@cornell.edu.
Isabella Garcia CaffaroCentro de Estudios en Biotecnología (CEB) Affiliated to the Centro de Estudios en Salud, Universidad del Valle de Guatemala (UVG), Guatemala, Guatemala.
Gabriela O Paiva-SilvaPrograma de Biologia Molecular e Biotecnologia, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Pedro L OliveiraPrograma de Biologia Molecular e Biotecnologia, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Pedro A RendonInternational Atomic Energy Agency, Technical Cooperation Projects for the Region of Latin America and the Caribbean, IAEA/TC-LAC - USDA/APHIS - Moscamed Program, Guatemala, Guatemala.
Ellen M DotsonDivision of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention (CDC), Center for Global Health, Atlanta, GA, USA.
Pamela M PenningtonCentro de Estudios en Biotecnología (CEB) Affiliated to the Centro de Estudios en Salud, Universidad del Valle de Guatemala (UVG), Guatemala, Guatemala.
Universidad del Valle de Guatemala · GTUniversidade Federal do Rio de Janeiro · BRCenters for Disease Control and Prevention · UGCornell University · USInternational Atomic Energy Agency · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA interference (RNAi) is a powerful mechanism that can be exploited not only for physiology research but also for designing insect pest management approaches. Some insects cause harm by vectoring diseases dangerous to humans, livestock, or plants or by damaging crops. For at least a decade now, different insect control strategies that induce RNAi by delivering double stranded RNA (dsRNA) targeting essential genes have been proposed. Here, we focus on nontransgenic RNAi-based approaches that use oral delivery of dsRNA through feeding of inactivated bacteria to produce RNAi in disease vectors and in a crop pest. This potential pest management method could be easily adapted to target different genes or similar organisms.

Indexed as

Escherichia coliAnimalsHumansInsectaInsect ControlRNA, Double-StrandedRNA InterferenceRNA, Double-StrandedNontransgenicOral deliveryReal-time PCRRNAi

Identifiers

PMID34495521
OpenAlexW3197123293

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.