ArticleDevelopment (Cambridge, England)2021
Knock-in tagging in zebrafish facilitated by insertion into non-coding regions.
Article in Development (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 53 citations in OpenAlex.
- Interfering with DNA repair pathways to enhance CRISPR-Cas9-mediated homology-directed repair in a chelicerate genetic model.iScience · 2026Article
- Zebrafish knock-in lines enabling live visualization of extracellular matrix dynamics during development and regeneration.Development (Cambridge, England) · 2026Article
- Hydrostatic pressure shapes and canalizes semicircular canal morphology to ensure vestibular function.bioRxiv : the preprint server for biology · 2026Article
- Dynamic interactions between epithelial skin cells and a sensory cavity sculpt the growing olfactory orifice.Nature communications · 2026Article
- Optimised genome editing for precise DNA insertion and substitution using prime editors in zebrafish.eLife · 2026Article
- Chronic exposure to low levels of glyphosate and metals induces kidney dysfunction.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- Loss of meningeal lymphatic endothelial cells results in formation of a newly forming vascular network with scavenging function.Development (Cambridge, England) · 2026Article
- Advancing knock-in approaches for robust genome editing in zebrafish.Biology open · 2026Article
- Strategies and considerations for the generation of ssDNA-Based HDR templates for CRISPR-based genome editing.BMC genomics · 2026Review
- RNA-binding protein Rbm38 as a multifaceted post-transcriptional regulator in zebrafish pancreatic development.Journal of molecular cell biology · 2026Article
- Harnessing population genetics and animal models to uncover the genetics of skin scarring.Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2026Review
- Advancing Knock-In Approaches for Robust Genome Editing in Zebrafish.bioRxiv : the preprint server for biology · 2025Article
- A self-limiting mechanotransduction feedback loop ensures robust organ formation.bioRxiv : the preprint server for biology · 2025Article
- Live visualization of extracellular matrix dynamics during development and regeneration in zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Nr4a1 modulates inflammation and heart regeneration in zebrafish.Development (Cambridge, England) · 2025Article
- Tissue-wide, synchronous Erk oscillations time the segmentation of the zebrafish notochord.bioRxiv : the preprint server for biology · 2025Article
- Cloning-Free Targeting of Endogenous Loci to Generate Fluorescent Reporters in Medaka.Bio-protocol · 2025Article
- Fluorescent Tagging of Endogenous FOXO for Live Imaging and Pull-Down Assays.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Initiation of lumen formation from junctions via differential actomyosin contractility regulated by dynamic recruitment of Rasip1.Nature communications · 2024Article
- Oscillatory contractile forces refine endothelial cell-cell interactions for continuous lumen formation governed by Heg1/Ccm1.Angiogenesis · 2024Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Zebrafish provide an excellent model for in vivo cell biology studies because of their amenability to live imaging. Protein visualization in zebrafish has traditionally relied on overexpression of fluorescently tagged proteins from heterologous promoters, making it difficult to recapitulate endogenous expression patterns and protein function. One way to circumvent this problem is to tag the proteins by modifying their endogenous genomic loci. Such an approach is not widely available to zebrafish researchers because of inefficient homologous recombination and the error-prone nature of targeted integration in zebrafish. Here, we report a simple approach for tagging proteins in zebrafish on their N or C termini with fluorescent proteins by inserting PCR-generated donor amplicons into non-coding regions of the corresponding genes. Using this approach, we generated endogenously tagged alleles for several genes that are crucial for epithelial biology and organ development, including the tight junction components ZO-1 and Cldn15la, the trafficking effector Rab11a, the apical polarity protein aPKC and the ECM receptor Integrin β1b. Our approach facilitates the generation of knock-in lines in zebrafish, opening the way for accurate quantitative imaging studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.