Evidence map›Paper›PMID 34494426›Full record

ArticleACS sensors2021

Nanoplasmonic Sandwich Immunoassay for Tumor-Derived Exosome Detection and Exosomal PD-L1 Profiling.

Chuanyu Wang, Chung-Hui Huang, Zhuangqiang Gao, Jialiang Shen, Jiacheng He, Alana MacLachlan, Chao Ma, Ya Chang, Wen Yang, Yuxin Cai and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS sensors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 79 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Chuanyu WangMaterials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.
Chung-Hui HuangDepartment of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, Auburn, Alabama 36849, United States.
Zhuangqiang GaoMaterials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.ORCID 0000-0001-9097-1799
Jialiang ShenMaterials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.
Jiacheng HeMaterials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.
Alana MacLachlanMaterials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.
Chao MaDepartment of Mechanical and Aerospace Engineering, New York University, Brooklyn, New York 11201, United States.
Ya ChangDepartment of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, Auburn, Alabama 36849, United States.
Wen YangMaterials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.
Yuxin CaiMaterials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.
Yang LouYurogen Biosystems LLC, Worcester, Massachusetts 01605, United States.
Siyuan DaiMaterials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.ORCID 0000-0001-7259-7182
Weiqiang ChenDepartment of Mechanical and Aerospace Engineering, New York University, Brooklyn, New York 11201, United States.ORCID 0000-0002-9469-8328
Feng LiDepartment of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, Auburn, Alabama 36849, United States.ORCID 0000-0002-8559-3831
Pengyu ChenMaterials Research and Education Center, Materials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.ORCID 0000-0003-3380-872X
Auburn University · USNew York University · US

Funding

Purchase of a modularized confocal microscopeR35GM133795 · NIGMS · AUBURN UNIVERSITY AT AUBURN · PI Pengyu Chen · 2019 to 2026
$3.3M
Purchase of spinning disk confocal microscopeR35GM133646 · NIGMS · NEW YORK UNIVERSITY · PI CHEN, WEIQIANG · 2019 to 2023
$2.0M
NIGMS NIH HHS R35 GM133646NIGMS NIH HHS R35 GM133795
6 · The paper itself

Abstract

Tumor-derived exosomes play a vital role in the process of cancer development. Quantitative analysis of exosomes and exosome-shuttled proteins would be of immense value in understanding cancer progression and generating reliable predictive biomarkers for cancer diagnosis and treatment. Recent studies have indicated the critical role of exosomal programmed death ligand 1 (PD-L1) in immune checkpoint therapy and its application as a patient stratification biomarker in cancer immunotherapy. Here, we present a nanoplasmonic exosome immunoassay utilizing gold-silver (Au@Ag) core-shell nanobipyramids and gold nanorods, which form sandwich immune complexes with target exosomes. The immunoassay generates a distinct plasmonic signal pattern unique to exosomes with specific exosomal PD-L1 expression, allowing rapid, highly sensitive exosome detection and accurate identification of PD-L1 exosome subtypes in a single assay. The developed nanoplasmonic sandwich immunoassay provides a novel and viable approach for tumor cell-derived exosome detection and analysis with quantitative molecular details of key exosomal proteins, manifesting its great potential as a transformative diagnostic tool for early cancer detection, prognosis, and post-treatment monitoring.

Indexed as

B7-H1 AntigenExosomesEarly Detection of CancerHumansImmunoassayNanotechnologyNeoplasmsB7-H1 Antigenexosomal proteinexosomesgold−silver core−shell nanobipyramids (Au@Ag NBPs)immunoassaylocalized surface plasmon resonance (LSPR)microfluidicsprogrammed death ligand 1 (PD-L1)

Identifiers

PMID34494426
PMCPMC9275046
OpenAlexW3198185300

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.