Evidence map›Paper›PMID 34494123›Full record

ArticleAnalytical and bioanalytical chemistry2021

Simultaneous analysis of PAH urinary mono- and dihydroxylated metabolites by GC-MS-MS following SPE and two-stage derivatization.

Marie Marques, Anne Maitre, Luc Choisnard, Christine Demeilliers, Renaud Persoons

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Marie MarquesUniversité Grenoble Alpes, CNRS Grenoble INP, CHU Grenoble Alpes, TIMC, 38000, Grenoble, France.
Anne MaitreUniversité Grenoble Alpes, CNRS Grenoble INP, CHU Grenoble Alpes, TIMC, 38000, Grenoble, France.
Luc ChoisnardDépartement de Pharmachimie Moléculaire, UMR CNRS 5063, Université Grenoble Alpes, 38000, Grenoble, France.
Christine DemeilliersUniversité Grenoble Alpes, CNRS Grenoble INP, CHU Grenoble Alpes, TIMC, 38000, Grenoble, France.
Renaud PersoonsUniversité Grenoble Alpes, CNRS Grenoble INP, CHU Grenoble Alpes, TIMC, 38000, Grenoble, France. RPersoons@chu-grenoble.fr.
Translational Innovation in Medicine and Complexity · FRCentre Hospitalier Universitaire de Grenoble · FRDépartement de Pharmacochimie Moléculaire · FRUniversité Grenoble Alpes · FR

Funding

Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail Project n° 2014-1-176
6 · The paper itself

Abstract

A new gas chromatography-tandem mass spectrometry method for the determination of mono- and dihydroxylated polycyclic aromatic hydrocarbon metabolites (OH-PAHs and diol-PAHs) in urine was developed and validated. Various sample preparation procedures were compared, namely liquid-liquid extraction (LLE), dispersive solid-phase extraction (dSPE), and SPE, alone or combined. A novel two-stage derivatization approach using 2 silylation reagents was developed, and an experimental procedure design was used to optimize the programmed temperature vaporization-solvent vent injection (PTV-SV) GC parameters. The method focused on 11 target compounds resulting from four- to five-ring suspected carcinogenic PAHs. SPE was identified as an acceptable and more convenient extraction method for all tested metabolites, with extraction rates ranging from 63 to 86% and relative standard deviations lower than 20%. The two-stage derivatization approach successfully allowed first the derivatization of OH-PAHs by MTBSTFA (N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide) and then diol-PAHs by BSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) in a single run. The limits of quantification were in the range of 0.01-0.02 μg l

Indexed as

AluminumCalibrationEnvironmental PollutantsGas Chromatography-Mass SpectrometryHumansHydroxylationLiquid-Liquid ExtractionMetallurgyOccupational ExposurePolycyclic Aromatic HydrocarbonsReproducibility of ResultsSolid Phase ExtractionTandem Mass SpectrometryAluminumEnvironmental PollutantsPolycyclic Aromatic HydrocarbonsDerivatizationExtraction techniquesMono- and dihydroxylated metabolitesPolycyclic aromatic hydrocarbonsTandem mass spectrometryUrine

Identifiers

PMID34494123
OpenAlexW3196614904

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.