Evidence map›Paper›PMID 34494022›Full record

ArticlebioRxiv : the preprint server for biology2021

Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid and Potential Utility for SARS-CoV-2 Detection.

Xiaoyu Zhang, Venubabu Kotikam, Eriks Rozners, Brian P Callahan

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Xiaoyu ZhangDepartment of Chemistry, Binghamton University, the State University of New York, 4400 Vestal Parkway East, Binghamton, New York 13902, United States.
Venubabu KotikamDepartment of Chemistry, Binghamton University, the State University of New York, 4400 Vestal Parkway East, Binghamton, New York 13902, United States.
Eriks RoznersDepartment of Chemistry, Binghamton University, the State University of New York, 4400 Vestal Parkway East, Binghamton, New York 13902, United States.
Brian P CallahanDepartment of Chemistry, Binghamton University, the State University of New York, 4400 Vestal Parkway East, Binghamton, New York 13902, United States.

Funding

Chemical Approaches to Control the Function of Regulatory RNAsR35GM130207 · NIGMS · STATE UNIVERSITY OF NY,BINGHAMTON · PI ERIKS ROZNERS · 2019 to 2026
$3.7M
Structural Mechanisms of Hedgehog Autoprocessing in Physiology and DiseaseR01CA206592 · NCI · RENSSELAER POLYTECHNIC INSTITUTE · PI CALLAHAN, BRIAN PATRICK, WANG, CHUNYU · 2017 to 2021
$2.0M
Rapid SARS-CoV-2 Detection Using Amplicon Templated Reporter Enzyme AssemblyR03AI163907 · NIAID · STATE UNIVERSITY OF NY,BINGHAMTON · PI CALLAHAN, BRIAN PATRICK · 2021 to 2022
$157k
NCI NIH HHS R01 CA206592NIAID NIH HHS R03 AI163907NIGMS NIH HHS R35 GM130207
6 · The paper itself

Abstract

Enzymatic beacons, or E-beacons, are 1:1 bioconjugates of the nanoluciferase enzyme linked covalently at its C-terminus to hairpin forming DNA oligonucleotides equipped with a dark quencher. We prepared E-beacons biocatalytically using the promiscuous "hedgehog" protein-cholesterol ligase, HhC. Instead of cholesterol, HhC attached nanoluciferase site-specifically to mono-sterylated hairpin DNA, prepared in high yield by solid phase synthesis. We tested three potential E-beacon dark quenchers: Iowa Black, Onyx-A, and dabcyl. Prototype E-beacon carrying each of those quenchers provided sequence-specific nucleic acid sensing through turn-on bioluminescence. For practical application, we prepared dabcyl-quenched E-beacons for potential use in detecting the COVID-19 virus, SARS-CoV-2. Targeting the E484 codon of the SARS-CoV-2 Spike protein, E-beacons (80 × 10

Identifiers

PMID34494022
PMCPMC8423218

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.