ArticleScientific reports2021
Melanoma cells adopt features of both mesenchymal and amoeboid migration within confining channels.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- RIPK4 function interferes with melanoma cell adhesion and metastasis.Molecular oncology · 2026Article
- Macrophages migrate persistently and directionally upon entering 2D confinement in the presence of extracellular matrix.Biology open · 2025Article
- Mechanometabolism: recent findings on the intersection of cell adhesion, cell migration, and metabolism.American journal of physiology. Cell physiology · 2025Review
- Macrophages migrate persistently and directionally upon entering 2D confinement in the presence of extracellular matrix.bioRxiv : the preprint server for biology · 2025Article
- The activation of INF2 by Piezo1/CaCurrent biology : CB · 2025Article
- Live imaging in zebrafish reveals tissue-specific strategies for amoeboid migration.Development (Cambridge, England) · 2025Article
- Blebology: principles of bleb-based migration.Trends in cell biology · 2024Review
- New Insights into the Exosome-Induced Migration of Uveal Melanoma Cells and the Pre-Metastatic Niche Formation in the Liver.Cancers · 2024Article
- The amoeboid migration of monocytes in confining channels requires the local remodeling of the cortical actin cytoskeleton by cofilin-1.Scientific reports · 2024Article
- Membrane to cortex attachment determines different mechanical phenotypes in LGR5+ and LGR5- colorectal cancer cells.Nature communications · 2024Article
- The amoeboid migration of monocytes in confining channels requires the local remodeling of the cortical actin cytoskeleton by cofilin-1.bioRxiv : the preprint server for biology · 2024Article
- The activation of INF2 by Piezo1/CabioRxiv : the preprint server for biology · 2024Article
- The amoeboid migration of monocytes in confining channels requires the local remodeling of the cortical actin cytoskeleton by cofilin-1.Research square · 2023Article
- The mechanosensitive TRPV2 calcium channel promotes human melanoma invasiveness and metastatic potential.EMBO reports · 2023Article
- Folylpolyglutamate synthetase mRNA G-quadruplexes regulate its cell protrusion localization and enhance a cancer cell invasive phenotype upon folate repletion.BMC biology · 2023Article
- Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study.Frontiers in molecular medicine · 2023Article
- Push or pull: how cytoskeletal crosstalk facilitates nuclear movement through 3D environments.Physical biology · 2022Review
- Blebs-Formation, Regulation, Positioning, and Role in Amoeboid Cell Migration.Frontiers in cell and developmental biology · 2022Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For metastasis to occur, cancer cells must traverse a range of tissue environments. In part, this is accomplished by cells adjusting their migration mode to one that is best suited to the environment. Melanoma cells have been shown to be particularly plastic, frequently using both mesenchymal and amoeboid (bleb-based) modes of migration. It has been demonstrated that 2D confinement will promote the transition from mesenchymal to bleb-based migration. However, if melanoma cells similarly transition to bleb-based migration in response to 3D confinement, such as within narrow channels, is unknown. Here, using micro-fabricated channels, we demonstrate that metastatic, A375-M2, melanoma cells adopt features of both mesenchymal and bleb-based migration. In narrow (8 µm; height and width) channels coated with fibronectin, ~ 50% of melanoma cells were found to use either mesenchymal or bleb-based migration modes. In contrast, the inhibition of Src family kinases or coating channels with BSA, completely eliminated any features of mesenchymal migration. Detailed comparisons of migration parameters revealed that blebbing cells, particularly in the absence of adhesions, were faster than mesenchymal cells. In contrast to what has been previously shown under conditions of 2D confinement, pharmacologically inhibiting Arp2/3 promoted a fast filopodial-based mode of migration. Accordingly, we report that melanoma cells adopt a unique range of phenotypes under conditions of 3D confinement.
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