ArticlePLoS biology2021
RhoA- and Cdc42-induced antagonistic forces underlie symmetry breaking and spindle rotation in mouse oocytes.
Article in PLoS biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- DEPDC7 couples homologous chromosome segregation to asymmetric cytokinesis through recruiting ARP2/3 complex in oocyte meiosis.Cell communication and signaling : CCS · 2026Article
- From Cytoskeletal Remodeling to Oocyte Quality: The Emerging Role of Mechanics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Chromatin- and actin-mediated mitochondrial streaming leads to patterning of mitochondrial distribution in oocytes.Nature communications · 2026Article
- Ultrastructural Aspects of Oocyte Maturation in Dogs, with Comparative Insights from Cats: Current Evidence and Research Perspectives.Animals : an open access journal from MDPI · 2026Review
- Mevalonate metabolites boost aged oocyte quality through prenylation of small GTPases.Nature aging · 2025Article
- Cellular Responses to Nanoscale Topography Mediated Through the RhoA/ROCK Pathway.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Functions of RhoA in the female reproductive system.Reproductive and developmental medicine · 2025Review
- CaCurrent biology : CB · 2025Article
- Supra-second tracking and live-cell karyotyping reveal principles of mitotic chromosome dynamics.Nature cell biology · 2025Article
- An egg-sabotaging mechanism drives non-Mendelian transmission in mice.Current biology : CB · 2024Article
- Sirtuin 5-driven meiotic spindle assembly and actin-based migration in mouse oocyte meiosis.Heliyon · 2024Article
- NLRP4E regulates actin cap formation through SRC and CDC42 during oocyte meiosis.Cellular & molecular biology letters · 2024Article
- An egg sabotaging mechanism drives non-Mendelian transmission in mice.bioRxiv : the preprint server for biology · 2024Article
- MRCK ensures cortex-chromatin "social distancing" to enable egg spindle rotation.The Journal of cell biology · 2023Article
- MRCK activates mouse oocyte myosin II for spindle rotation and male pronucleus centration.The Journal of cell biology · 2023Article
- Cell fragmentation in mouse preimplantation embryos induced by ectopic activation of the polar body extrusion pathway.The EMBO journal · 2023Article
- Meiotic drive of noncentromeric loci in mammalian meiosis II eggs.Current opinion in genetics & development · 2023Review
- Bypassing Mendel's First Law: Transmission Ratio Distortion in Mammals.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian oocyte meiotic divisions are highly asymmetric and produce a large haploid gamete and 2 small polar bodies. This relies on the ability of the cell to break symmetry and position its spindle close to the cortex before anaphase occurs. In metaphase II-arrested mouse oocytes, the spindle is actively maintained close and parallel to the cortex, until fertilization triggers sister chromatid segregation and the rotation of the spindle. The latter must indeed reorient perpendicular to the cortex to enable cytokinesis ring closure at the base of the polar body. However, the mechanisms underlying symmetry breaking and spindle rotation have remained elusive. In this study, we show that spindle rotation results from 2 antagonistic forces. First, an inward contraction of the cytokinesis furrow dependent on RhoA signaling, and second, an outward attraction exerted on both sets of chromatids by a Ran/Cdc42-dependent polarization of the actomyosin cortex. By combining live segmentation and tracking with numerical modeling, we demonstrate that this configuration becomes unstable as the ingression progresses. This leads to spontaneous symmetry breaking, which implies that neither the rotation direction nor the set of chromatids that eventually gets discarded are biologically predetermined.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.