ArticleAnalytical and bioanalytical chemistry2021
Fast label-free identification of bacteria by synchronous fluorescence of amino acids.
Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 20 citations in OpenAlex.
- Application of process analytical technology for real-time monitoring of synthetic co-culture bioprocesses.Analytical and bioanalytical chemistry · 2025Review
- Fluorescence-based spectrometric and imaging methods and machine learning analyses for microbiota analysis.Mikrochimica acta · 2025Review
- Optimizing Excitation Light for Accurate Rapid Bacterial Species Identification with Autofluorescence.Journal of fluorescence · 2024Article
- Nanostructured biosensing platforms for the detection of food- and water-borne pathogenic Escherichia coli.Analytical and bioanalytical chemistry · 2023Review
- Non-photosynthetic bacteria produce photocurrent mediated by NADH.bioRxiv : the preprint server for biology · 2023Article
- Advantages of imprinted polymer electrodes for electrochemical pathogen detection.Current opinion in electrochemistry · 2022Article
- Direct Electricity Production fromInternational journal of molecular sciences · 2022Article
- Identification of bacteria by poly-aromatic hydrocarbon biosensors.Analytical and bioanalytical chemistry · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fast identification of pathogenic bacteria is an essential need for patient's diagnostic in hospitals and environmental monitoring of water and air quality. Bacterial cells consist of a very high amount of biological molecules whose content changes in response to different environmental conditions. The similarity between the molecular compositions of different bacterial cells limits the possibility to find unique markers to enable differentiation among species. Although many biological molecules in the cells absorb at the UV-Vis region, only a few of them can be detected in whole cells by their intrinsic fluorescence. Among these molecules are the amino acids phenylalanine, tyrosine, and tryptophan. In this work, we develop a rapid method for bacterial identification by synchronous fluorescence. We show that we can quantify the concentration for the 3 amino acids without any significant interference from other fluorophores in the cells and that we can differentiate among 6 pathogenic bacterial species by using the concentrations of their amino acids as a bacterial fingerprint. Fluorescent amino acids exist in all living cells. Therefore, this method has the potential to be applicative for the rapid identification of cells from all kinds of organisms.
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