ArticleAngewandte Chemie (International ed. in English)2021
Engineering Fluorophore Recycling in a Fluorogenic RNA Aptamer.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 37 citations in OpenAlex.
- Non-Covalent Reversibly Photoconvertible Fluorescent Tags for Wash-Free Protein Labeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Programmable artificial RNA condensates in mammalian cells.Nature nanotechnology · 2026Article
- Expression of nano-engineered RNA organelles in bacteria.Nature communications · 2026Article
- Programmable artificial RNA condensates in mammalian cells.bioRxiv : the preprint server for biology · 2026Article
- Near-infrared fluorogenic RNA for in vivo imaging and sensing.Nature communications · 2025Article
- Fluorogenic CRISPR for genomic DNA imaging.Nature communications · 2024Article
- Article
- Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells.Nature methods · 2023Article
- Monitoring amyloid aggregationChemical science · 2023Article
- Engineering fluorescent protein chromophores with an internal reference for high-fidelity ratiometric G4 imaging in living cells.Chemical science · 2023Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Fluorogenic aptamers can potentially show minimal photobleaching during continuous irradiation since any photobleached fluorophore can exchange with fluorescent dyes in the media. However, fluorophores have not been designed to maximize "fluorophore recycling." Here we describe TBI, a novel fluorophore for the Broccoli fluorogenic aptamer. Previous fluorophores either fail to rapidly dissociate when they undergo photobleaching via cis-trans isomerization, or bind slowly, resulting in extended periods after dissociation of the photobleached fluorophore when no fluorophore is bound. By contrast, photobleached TBI dissociates rapidly from Broccoli, and TBI from the media rapidly replaces dissociated photobleached fluorophore. Using TBI, Broccoli exhibits markedly enhanced fluorescence in cells during continuous imaging. These data show that designing fluorophores to optimize fluorophore recycling can lead to enhanced fluorescence of fluorogenic aptamers.
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Registered trials
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