Evidence map›Paper›PMID 34489427›Full record

ArticleNature communications2021

Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production.

Annica Saaret, Benoît Villiers, François Stricher, Macha Anissimova, Mélodie Cadillon, Reynard Spiess, Sam Hay, David Leys

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Annica SaaretDepartment of Chemistry, Manchester Institute for Biotechnology, University of Manchester, Manchester, UK.ORCID 0000-0001-6315-6537
Benoît VilliersGlobal Bioenergies, Evry-Courcouronnes, France. benoit.villiers@global-bioenergies.com.
François StricherGlobal Bioenergies, Evry-Courcouronnes, France.ORCID 0000-0003-0150-0878
Macha AnissimovaGlobal Bioenergies, Evry-Courcouronnes, France.ORCID 0000-0003-0552-8157
Mélodie CadillonGlobal Bioenergies, Evry-Courcouronnes, France.
Reynard SpiessDepartment of Chemistry, Manchester Institute for Biotechnology, University of Manchester, Manchester, UK.
Sam HayDepartment of Chemistry, Manchester Institute for Biotechnology, University of Manchester, Manchester, UK.ORCID 0000-0003-3274-0938
David LeysDepartment of Chemistry, Manchester Institute for Biotechnology, University of Manchester, Manchester, UK. david.leys@manchester.ac.uk.ORCID 0000-0003-4845-8443
University of Manchester · GBTerritoires, Villes, Environnement & Société · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isobutene is a high value gaseous alkene used as fuel additive and a chemical building block. As an alternative to fossil fuel derived isobutene, we here develop a modified mevalonate pathway for the production of isobutene from glucose in vivo. The final step in the pathway consists of the decarboxylation of 3-methylcrotonic acid, catalysed by an evolved ferulic acid decarboxylase (Fdc) enzyme. Fdc belongs to the prFMN-dependent UbiD enzyme family that catalyses reversible decarboxylation of (hetero)aromatic acids or acrylic acids with extended conjugation. Following a screen of an Fdc library for inherent 3-methylcrotonic acid decarboxylase activity, directed evolution yields variants with up to an 80-fold increase in activity. Crystal structures of the evolved variants reveal that changes in the substrate binding pocket are responsible for increased selectivity. Solution and computational studies suggest that isobutene cycloelimination is rate limiting and strictly dependent on presence of the 3-methyl group.

Indexed as

AlkenesBiocatalysisCarboxy-LyasesCrotonatesDirected Molecular EvolutionEscherichia coliEscherichia coli ProteinsFermentationFlavin MononucleotideGlucoseHypocrealesMevalonic AcidPrenylationAlkenesCarboxy-LyasesCrotonatescrotonic acidEscherichia coli ProteinsFlavin MononucleotideGlucoseisobutyleneMevalonic Acidphenylacrylic acid decarboxylaseubiX protein, E coli

Identifiers

PMID34489427
PMCPMC8421414
OpenAlexW3196312632

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.