Evidence map›Paper›PMID 34486645›Full record

ArticleBioscience reports2021

MiR-192-5p inhibits proliferation, migration, and invasion in papillary thyroid carcinoma cells by regulation of SH3RF3.

Songbo Fu, Chengxu Ma, Xulei Tang, Xiaoni Ma, Gaojing Jing, Nan Zhao, Juntao Ran

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PubMed Full text at PMC Publisher
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Article in Bioscience reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

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  8. A versatile genomic transgenesis platform with enhancedFrontiers in bioengineering and biotechnology · 2023
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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Songbo FuThe First Clinical Medical School, Lanzhou University, Lanzhou, Gansu 730000, China.ORCID 0000-0003-0611-6015
Chengxu MaThe First Clinical Medical School, Lanzhou University, Lanzhou, Gansu 730000, China.
Xulei TangThe First Clinical Medical School, Lanzhou University, Lanzhou, Gansu 730000, China.
Xiaoni MaDepartment of Endocrinology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, China.
Gaojing JingDepartment of Endocrinology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, China.
Nan ZhaoDepartment of Endocrinology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, China.
Juntao RanDepartment of Radiation Oncology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe decreased level of miR-192-5p has been reported in several kinds of cancers, including bladder, colon, ovarian, and non-small cell lung cancer. However, the expression and function of miR-192-5p in papillary thyroid carcinoma/cancer (PTC) remains unknown.

objectiveThe present study aimed to explore the function and underlying mechanism of miR-192-5p in PTC development.

methodsPTC tissues and relative normal controls from PTC patients were collected. qRT-PCR analysis was performed to measure miR-192-5p and SH3RF3 mRNA level in PTC tissues and cell lines. CCK-8 method and FCM assay were used to test cell proliferation and apoptosis in TPC-1 cells, respectively. The abilities of cell migration and invasion were detected by wound healing and transwell assays, respectively. The protein expression was evaluated by Western blot. The interaction between miR-192-5p and Src homology 3 (SH3) domain containing ring finger 3 (SH3RF3) were confirmed by dual-luciferase reporter assay.

resultsMiR-192-5p level was obviously decreased in PTC tissues and cell lines. Overexpression of miR-192-5p suppressed proliferation, migration, invasion, and EMT process, while induced apoptosis in TPC-1 cells. In addition, miR-192-5p negatively modulated SH3RF3 expression by binding to its 3'-untranslated region (3'UTR). Silencing SH3RF3 inhibited the migration, invasion, and EMT of TPC-1 cells. In the meantime, matrine, an alkaloid extracted from herb, exerted its anti-cancer effects in PTC cells dependent on increase in miR-192-5p expression and decrease in SH3RF3 expression.

conclusionWe firstly declared that miR-192-5p played a tumor suppressive role in PTC via targeting SH3RF3. Moreover, matrine exerted its anti-cancer effects in PTC via regulating miR-192-5p/SH3RF3 pathway.

Indexed as

Cell MovementCell ProliferationAlkaloidsAntineoplastic Agents, PhytogenicApoptosisCell Line, TumorEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticHumansMatrinesMicroRNAsNeoplasm InvasivenessQuinolizinesSignal TransductionThyroid Cancer, PapillaryThyroid NeoplasmsAlkaloidsAntineoplastic Agents, PhytogenicMatrinesMicroRNAsMIRN192 microRNA, humanQuinolizinesSH3RF3 protein, humanUbiquitin-Protein LigasesmicroRNA-192-5ppapillary thyroid cancerSH3RF3

Identifiers

PMID34486645
PMCPMC8463656

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.