Evidence map›Paper›PMID 34486244›Full record

ArticleAdvanced healthcare materials2021

Sulfated Alginate Hydrogels Prolong the Therapeutic Potential of MSC Spheroids by Sequestering the Secretome.

Marissa Gionet-Gonzales, Alena Casella, Daphne Diloretto, Clara Ginnell, Katherine H Griffin, Anne Bigot, J Kent Leach

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 49 citations in OpenAlex.

  1. Article
  2. Edible Sulfonated Soy Protein Microcarriers for Cultivated Meat Cell Expansion.Journal of biomedical materials research. Part A · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Marissa Gionet-GonzalesDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, 95616, USA.
Alena CasellaDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, 95616, USA.
Daphne DilorettoDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, 95616, USA.
Clara GinnellDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, 95616, USA.
Katherine H GriffinSchool of Veterinary Medicine, University of California, Davis, Davis, CA, 95616, USA.
Anne BigotUniversite de Paris, Institut de Myologie, Paris, 75013, France.
J Kent LeachDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, 95616, USA.ORCID 0000-0002-1673-3335
University of California, Davis · USUC Davis Health · USUniversité Paris Cité · FR

Funding

Dual peptide presentation from bioengineered carriers to potentiate stromal cell function and tissue repairR01DE025899 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEACH, J. KENT · 2017 to 2021
$2.5M
Identifying the superior ossification pathway for tissue engineered approaches to long bone repairR01AR079211 · NIAMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEACH, J. KENT · 2021 to 2025
$1.9M
Biomaterial regulation of cell spheroids to synergistically enhance bone healingR01DE025475 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEACH, J. KENT · 2015 to 2019
$1.9M
NIAMS NIH HHS R01 AR079211NIDCR NIH HHS R01 DE025475NIDCR NIH HHS R01 DE025899
6 · The paper itself

Abstract

Cell-based approaches to tissue repair suffer from rapid cell death upon implantation, limiting the window for therapeutic intervention. Despite robust lineage-specific differentiation potential in vitro, the function of transplanted mesenchymal stromal cells (MSCs) in vivo is largely attributed to their potent secretome comprising a variety of growth factors (GFs). Furthermore, GF secretion is markedly increased when MSCs are formed into spheroids. Native GFs are sequestered within the extracellular matrix (ECM) via sulfated glycosaminoglycans, increasing the potency of GF signaling compared to their unbound form. To address the critical need to prolong the efficacy of transplanted cells, alginate hydrogels are modified with sulfate groups to sequester endogenous heparin-binding GFs secreted by MSC spheroids. The influence of crosslinking method and alginate modification is assessed on mechanical properties, degradation rate, and degree of sulfate modification. Sulfated alginate hydrogels sequester a mixture of MSC-secreted endogenous biomolecules, thereby prolonging the therapeutic effect of MSC spheroids for tissue regeneration. GFs are sequestered for longer durations within sulfated hydrogels and retain their bioactivity to regulate endothelial cell tubulogenesis and myoblast infiltration. This platform has the potential to prolong the therapeutic benefit of the MSC secretome and serve as a valuable tool for investigating GF sequestration.

Indexed as

HydrogelsMesenchymal Stem CellsAlginatesSpheroids, CellularSulfatesAlginatesHydrogelsSulfatesheparin-bindingmesenchymal stromal cellssecretomespheroidssulfate

Identifiers

PMID34486244
PMCPMC8568671
OpenAlexW3196904578

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.