ArticleACS photonics2021
2.5D microscopy: Fast, high-throughput imaging via volumetric projection for quantitative subcellular analysis.
Article in ACS photonics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Cytoskeletal Prestress Regulates RIG-I-Mediated Innate Immunity.Biophysica · 2025Article
- Multiplane 2.5D microscopy for high-throughput high-resolution tissue imaging.Journal of biomedical optics · 2025Article
- Photonic lantern TIRF microscopy for highly efficient, uniform, artifact-free imaging.Optics express · 2024Article
- Highly sensitive volumetric single-molecule imaging.Nanophotonics (Berlin, Germany) · 2024Article
- A comparison of confocal and epifluorescence microscopy for quantification of RNAScope and immunohistochemistry fluorescent images.Journal of neuroscience methods · 2024Article
- Video-rate 3D imaging of living cells using Fourier view-channel-depth light field microscopy.Communications biology · 2023Article
- E2E-BPF microscope: extended depth-of-field microscopy using learning-based implementation of binary phase filter and image deconvolution.Light, science & applications · 2023Article
- Pupil engineering for extended depth-of-field imaging in a fluorescence miniscope.Neurophotonics · 2023Article
- Large depth-of-field ultra-compact microscope by progressive optimization and deep learning.Nature communications · 2023Article
- 2.5D microscopy with polarization independent SLM for enhanced detection efficiency and aberration correction.Optics express · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Imaging-based single-cell analysis is essential to study the expression level and functions of biomolecules at subcellular resolution. However, its low throughput has prevented the measurement of numerous cellular features from multiples cells in a rapid and efficient manner. Here we report 2.5D microscopy that significantly improves the throughput of fluorescence imaging systems while maintaining high-resolution and single-molecule sensitivity. Instead of sequential z-scanning, volumetric information is projected onto a 2D image plane in a single shot by engineering the emitted fluorescence light. Our approach provides an improved imaging speed and uniform focal response within a specific imaging depth, which enabled us to perform quantitative single-molecule RNA measurements over a 2×2 mm
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