ArticleAnalytica chimica acta2021
A lab-on-a-chip platform for integrated extraction and detection of SARS-CoV-2 RNA in resource-limited settings.
Article in Analytica chimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Integrated approach for detection of SARS-CoV-2 and its variant by utilizing LAMP and ARMS-PCR.Annals of clinical microbiology and antimicrobials · 2024Article
- Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review.Biosensors · 2023Review
- Addressing the challenges of establishing quality wastewater or non-sewered sanitation-based surveillance, including laboratory and epidemiological considerations, in Malawi.BMJ global health · 2023Article
- Quantitative analysis of respiratory viruses based on lab-on-a-chip platform.Analytical and bioanalytical chemistry · 2023Article
- Point-of-Care Devices for Viral Detection: COVID-19 Pandemic and Beyond.Micromachines · 2023Review
- Integrated microscale immiscible phase extraction and isothermal amplification for colorimetric detection of Neisseria gonorrhoeae.Analytical and bioanalytical chemistry · 2023Article
- Molecular test for COVID-19 diagnosis based on a colorimetric genomagnetic assay.Analytica chimica acta · 2023Article
- Article
- Microfluidic-based technologies for diagnosis, prevention, and treatment of COVID-19: recent advances and future directions.Biomedical microdevices · 2023Review
- A sample-to-answer COVID-19 diagnostic device based on immiscible filtration and CRISPR-Cas12a-assisted detection.Talanta open · 2022Article
- Integration of RT-LAMP and Microfluidic Technology for Detection of SARS-CoV-2 in Wastewater as an Advanced Point-of-Care Platform.Food and environmental virology · 2022Article
- LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.Biosensors · 2022Review
- A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics.Microchemical journal : devoted to the application of microtechniques in all branches of science · 2022Article
- Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2.Micromachines · 2022Review
- Microfluidics-based strategies for molecular diagnostics of infectious diseases.Military Medical Research · 2022Review
- Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives.Biomolecules · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the unprecedented global pandemic of coronavirus disease-2019 (COVID-19). Efforts are needed to develop rapid and accurate diagnostic tools for extensive testing, allowing for effective containment of the infection via timely identification and isolation of SARS-CoV-2 carriers. Current gold standard nucleic acid tests require many separate steps that need trained personnel to operate specialist instrumentation in laboratory environments, hampering turnaround time and test accessibility, especially in low-resource settings. We devised an integrated on-chip platform coupling RNA extraction based on immiscible filtration assisted by surface tension (IFAST), with RNA amplification and detection via colorimetric reverse-transcription loop mediated isothermal amplification (RT-LAMP), using two sets of primers targeting open reading frame 1a (ORF1a) and nucleoprotein (N) genes of SARS-CoV-2. Results were identified visually, with a colour change from pink to yellow indicating positive amplification, and further confirmed by DNA gel electrophoresis. The specificity of the assay was tested against HCoV-OC43 and H1N1 RNAs. The assay based on use of gene N primers was 100% specific to SARS-CoV-2 with no cross-reactivity to HCoV-OC43 nor H1N1. Proof-of-concept studies on water and artificial sputum containing genomic SARS-CoV-2 RNA showed our IFAST RT-LAMP device to be capable of extracting and detecting 470 SARS-CoV-2 copies mL
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.