ArticleFish physiology and biochemistry2021
Screening of reference genes in tiger puffer (Takifugu rubripes) across tissues and under different nutritional conditions.
Article in Fish physiology and biochemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 13 citations in OpenAlex.
- Effects of Different Monomeric Bile Acids in the Diet on the Growth and Lipid Metabolism of Juvenile Pacific White Shrimp.Animals : an open access journal from MDPI · 2026Article
- Comparative evaluation of four reference genes for qRT-PCR assays in snow trout (Schizothorax richardsonii) under multiple experimental conditions.Scientific reports · 2026Article
- Molecular characterization and function of hif1a and fih1 in response to acute thermal stress in American shad (Alosa sapidissima).Fish physiology and biochemistry · 2024Article
- Systematic selection of suitable reference genes for quantitative real-time PCR normalization studies of gene expression in Lutjanus erythropterus.Scientific reports · 2024Article
- Dietary Lysophosphatidylcholine Improves the Uptake of Astaxanthin and Modulates Cholesterol Transport in Pacific White ShrimpAntioxidants (Basel, Switzerland) · 2024Article
- Efficacy of Palm Oil Application in Tiger Puffer Diets: Growth, Body Composition, Muscle Texture, and Lipid Metabolism.Aquaculture nutrition · 2024Article
- Evaluation of qPCR reference genes for taimen (Hucho taimen) under heat stress.Scientific reports · 2022Article
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Authors and funding
8 authors at 3 institutions in 1 country.
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Abstract
The present study was aimed at screening suitable reference genes for quantitative real-time polymerase chain reaction (qRT-PCR) in tiger puffer (Takifugu rubripes), an important aquaculture species in Asia and also a good model species for lipid research. Specifically, this reference gene screening was targeted at standardization of gene expression in different tissues (liver, muscle, brain, intestine, heart, eye, skin, and spleen) or under different nutritional conditions (starvation and different dietary lipid levels). Eight candidate reference genes (ribosomal protein L19 and L13 (RPL19 and RPL13), elongation factor-1 alpha (EF1α), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), hypoxanthine guanine phosphoribosyl transferase1 (HPRT1), beta-2-Microglobulin (B2M), 18S ribosomal RNA (18SrRNA), and beta actin (ACTB)) were evaluated with four algorithms (geNorm, NormFinder, BestKeeper, and comparative ΔCt method). The results showed that different algorithms generated inconsistent results. Based on these findings, RPL19, EF1α, 18SrRNA, and RPL13 were relatively stable in different tissues of tiger puffer. During starvation conditions, ACTB/RPL19 was the best reference gene combination. Under different dietary lipid levels, ACTB/RPL13 was the most suitable reference gene combination. The present results will help researchers to obtain more accurate results in future qRT-PCR analysis in tiger puffer.
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