ReviewNature protocols2021
Hierarchical imaging and computational analysis of three-dimensional vascular network architecture in the entire postnatal and adult mouse brain.
Review in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 56 citations in OpenAlex.
- Analytical model enabling the correction of absorption and scattering in dual-color ratiometric multiphoton microscopy of brain tissue.Neurophotonics · 2026Article
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- CD98hc controls CNS angiogenesis and blood-brain barrier integrity through localized regulation of the systemic integrin-FAK pathway.Nature cardiovascular research · 2026Article
- A high-resolution dataset of mouse brain vasculature for deep learning-based reconstruction.Frontiers in neuroinformatics · 2026Article
- Hypoxic-ischemic brain injury in neonatal mice sequentially recruits neutrophils with dichotomous phenotype and function.Nature communications · 2025Article
- Vasomotion delineates cerebral vascular dynamic features and participates in the homeostatic cerebral blood flow regulation.Scientific reports · 2025Article
- Genetic tools for imaging microcirculation via plasma labeling.Anatomical science international · 2025Review
- Advances in tissue optical clearing for 3D imaging in large animal.Frontiers of optoelectronics · 2025Review
- Capturing the Electrical Activity of all Cortical Neurons: Are Solutions Within Reach?Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Dissecting endothelial cell heterogeneity with new tools.Cell regeneration (London, England) · 2025Review
- Renal microcirculation and mechanisms in diabetic kidney disease.Frontiers in endocrinology · 2025Review
- Depth-dependent contributions of various vascular zones to cerebral autoregulation and functional hyperemia: An in-silico analysis.PloS one · 2025Article
- Self-supervised learning for accurately modelling hierarchical evolutionary patterns of cerebrovasculature.Nature communications · 2024Article
- ARViS: a bleed-free multi-site automated injection robot for accurate, fast, and dense delivery of virus to mouse and marmoset cerebral cortex.Nature communications · 2024Article
- Mouse Cardiovascular Imaging.Current protocols · 2024Review
- Single-cell atlas of the human brain vasculature across development, adulthood and disease.Nature · 2024Article
- Article
- Revealing the complexity of meniscus microvasculature through 3D visualization and analysis.Scientific reports · 2024Article
- Using digital pathology to analyze the murine cerebrovasculature.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024Article
- Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images.PloS one · 2024Article
Corrections and comments
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Authors and funding
12 authors at 8 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation of new blood vessels and the establishment of vascular networks are crucial during brain development, in the adult healthy brain, as well as in various diseases of the central nervous system. Here, we describe a step-by-step protocol for our recently developed method that enables hierarchical imaging and computational analysis of vascular networks in postnatal and adult mouse brains. The different stages of the procedure include resin-based vascular corrosion casting, scanning electron microscopy, synchrotron radiation and desktop microcomputed tomography imaging, and computational network analysis. Combining these methods enables detailed visualization and quantification of the 3D brain vasculature. Network features such as vascular volume fraction, branch point density, vessel diameter, length, tortuosity and directionality as well as extravascular distance can be obtained at any developmental stage from the early postnatal to the adult brain. This approach can be used to provide a detailed morphological atlas of the entire mouse brain vasculature at both the postnatal and the adult stage of development. Our protocol allows the characterization of brain vascular networks separately for capillaries and noncapillaries. The entire protocol, from mouse perfusion to vessel network analysis, takes ~10 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.