Evidence map›Paper›PMID 34479608›Full record

ArticleParasites & vectors2021

Molecular detection of Strongyloides sp. in Australian Thoroughbred foals.

Ghazanfar Abbas, Abdul Ghafar, Anson V Koehler, Jenni Bauquier, Edwina J A Wilkes, Caroline Jacobson, Anne Beasley, John Hurley, Lucy Cudmore, Peter Carrigan and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 11 citations in OpenAlex.

  1. Epidemiological study and risk factors of equine (Parasite epidemiology and control · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 5 institutions in 2 countries.

Ghazanfar AbbasMelbourne Veterinary School, The University of Melbourne, Werribee, Australia.
Abdul GhafarMelbourne Veterinary School, The University of Melbourne, Werribee, Australia.
Anson V KoehlerMelbourne Veterinary School, The University of Melbourne, Werribee, Australia.
Jenni BauquierMelbourne Veterinary School, The University of Melbourne, Werribee, Australia.
Edwina J A WilkesSchool of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, Australia.
Caroline JacobsonCentre for Animal Production and Health, Murdoch University, Murdoch, Australia.
Anne BeasleySchool of Agriculture and Food Science, University of Queensland, Gatton, Australia.
John HurleySwettenham Stud, Nagambie, Australia.
Lucy CudmoreScone Equine Hospital, Scone, Australia.
Peter CarriganScone Equine Hospital, Scone, Australia.
Brett Tennent-BrownMelbourne Veterinary School, The University of Melbourne, Werribee, Australia.
Charles El-HageMelbourne Veterinary School, The University of Melbourne, Werribee, Australia.
Martin K NielsenM.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.
Charles G GauciMelbourne Veterinary School, The University of Melbourne, Werribee, Australia.
Kristopher J HughesSchool of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, Australia.
Ian BeveridgeMelbourne Veterinary School, The University of Melbourne, Werribee, Australia.
Abdul JabbarMelbourne Veterinary School, The University of Melbourne, Werribee, Australia. jabbara@unimelb.edu.au.ORCID http://orcid.org/0000-0001-8888-0046
The University of Melbourne · AUCharles Sturt University · AUMurdoch University · AUThe University of Queensland · AUUniversity of Kentucky · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundStrongyloides westeri is found in the small intestine of young horses, mainly in foals up to about 16 weeks of age. The main source of infection for foals is through transmammary transmission, and foals can develop acute diarrhoea, weakness, dermatitis and respiratory signs. The epidemiology of S. westeri in Australia is largely unknown. Further, molecular techniques have never been employed for detection of S. westeri in horses. This pilot study aimed to assess the utility of a molecular phylogenetic method for the detection of S. westeri in the faeces of foals.

methodsFaecal samples were collected from a foal of less than 2 months of age, and eggs of Strongyloides sp. were detected using the modified McMaster technique. DNA was extracted from purified eggs, and a partial fragment of the small subunit of the nuclear ribosomal DNA (18S) was characterised using polymerase chain reaction, DNA sequencing and phylogenetic methods.

resultsMicroscopic examination of faeces revealed small ellipsoidal eggs typical of Strongyloides sp. The 18S sequence generated by PCR in this study revealed 98.4% identity with that of a reference sequence of S. westeri available from GenBank. Phylogenetic analyses revealed a polyphyletic clustering of S. westeri sequences.

conclusionThis is the first study reporting the detection of DNA of Strongyloides sp. in faeces of a foal using a molecular phylogenetic approach targeting the variable region of 18S rDNA. It is anticipated that this study will allow future molecular epidemiological studies on S. westeri in horses.

Indexed as

PhylogenyAge FactorsAnimalsAntiparasitic AgentsAustraliaBreedingDNA, HelminthFecesHorse DiseasesHorsesIvermectinParasite Egg CountPilot ProjectsStrongyloidesStrongyloidiasisAntiparasitic AgentsDNA, HelminthIvermectinAustralian Thoroughbred horsesGenetic characterisationSanger sequencingStrongyloides sp.

Identifiers

PMID34479608
PMCPMC8414860
OpenAlexW3198647889

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.