ArticlePlant methods2021
Genotyping of polyploid plants using quantitative PCR: application in the breeding of white-fleshed triploid loquats (Eriobotrya japonica).
Article in Plant methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 15 citations in OpenAlex.
- Genomic variation analysis and discovery of stable heterozygous loci in Loquat (Eriobotrya Japonica Lindl.).BMC plant biology · 2026Article
- A duplex sequencing approach for high-sensitivity detection of genome-edited plants.Food chemistry. Molecular sciences · 2025Article
- Occurrence of White Flesh Color and Refreshing Flavor FollowingJournal of agricultural and food chemistry · 2025Article
- Combination of GWAS and FMolecular genetics and genomics : MGG · 2023Article
- Photosynthetic Efficiency and Glyco-Metabolism Changes in Artificial Triploid Loquats Contribute to Heterosis Manifestation.International journal of molecular sciences · 2022Article
- Low Female Gametophyte Fertility Contributes to the Low Seed Formation of the Diploid Loquat [Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPloidy manipulation is effective in seedless loquat breeding, in which flesh color is a key agronomic and economic trait. Few techniques are currently available for detecting the genotypes of polyploids in plants, but this ability is essential for most genetic research and molecular breeding.
resultsWe developed a system for genotyping by quantitative PCR (qPCR) that allowed flesh color genotyping in multiple tetraploid and triploid loquat varieties (lines). The analysis of 13 different ratios of DNA mixtures between two homozygous diploids (AA and aa) showed that the proportion of allele A has a high correlation (R
conclusionsqPCR can detect the flesh color genotypes of loquat polyploids and provides an alternative method for analyzing polyploid genotype and breeding, dose effects and allele-specific expression.
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