Evidence map›Paper›PMID 34479492›Full record

ArticleBMC cancer2021

Head and neck squamous cell carcinoma cell lines have an immunomodulatory effect on macrophages independent of hypoxia and toll-like receptor 9.

Tamiko Ishizu, Dominik Eichin, Artur Padzik, Sanni Tuominen, Reidar Grénman, Marko Salmi, Tove J Grönroos, Johanna M Tuomela

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Tamiko IshizuInstitute of Biomedicine, University of Turku, Turku, Finland. tamish@utu.fi.
Dominik EichinMedicity Research Laboratory, University of Turku, Turku, Finland.
Artur PadzikTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Sanni TuominenInstitute of Biomedicine, University of Turku, Turku, Finland.
Reidar GrénmanDepartment of Otorhinolaryngology, University of Turku, Turku, Finland.
Marko SalmiInstitute of Biomedicine, University of Turku, Turku, Finland.
Tove J GrönroosMedicity Research Laboratory, University of Turku, Turku, Finland.
Johanna M TuomelaInstitute of Biomedicine, University of Turku, Turku, Finland.
University of Turku · FITurku Centre for Computer Science · FITurku PET Centre · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundA low tissue oxygen level, < 1% O

methodsConditioned media (CM

resultsOur results demonstrate that the soluble mediators produced by the cancer cells under normoxia polarize macrophages towards a hybridized M1/M2a/M2c phenotype. Furthermore, the results suggest that hypoxia has a limited role in altering the array of cancer-produced soluble factors affecting macrophage polarization and cytokine production. Our data also indicates that increased expression of TLR9 due to hypoxia in malignant cells does not markedly influence the polarization of macrophages. TLR9 transcriptional response to hypoxia is dissimilar to a HIF1-α-regulated LDH-A. This may indicate a context-dependent expression of TLR9 under hypoxia.

conclusionsHNSCC cell lines affect both macrophage activity (polarization) and functionality (cytokines), but with exception to iNOS expression, the effects appear independent of hypoxia and TLR9.

Indexed as

ImmunomodulationCell DifferentiationCytokinesHead and Neck NeoplasmsHumansHypoxiaMacrophage ActivationMacrophagesSquamous Cell Carcinoma of Head and NeckToll-Like Receptor 9Tumor Cells, CulturedTumor MicroenvironmentCytokinesTLR9 protein, humanToll-Like Receptor 9Anti-cancer immunomodulationHead and neck squamous cell carcinomaHypoxiaImmune evasionImmunoeditingInnate immune responseMacrophage polarizationTLR9

Identifiers

PMID34479492
PMCPMC8418007
OpenAlexW3197875893

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.