ArticleCell and tissue research2021
Expression and functional analysis of cytoplasmic dynein during spermatogenesis in Portunus trituberculatus.
Article in Cell and tissue research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Pt-LIS1 participates nuclear deformation and acrosome formation via regulating Dynein-1 during spermatogenesis in Portunus trituberculatus.Scientific reports · 2025Article
- Special Issue: Molecular Advance on Reproduction and Fertility of Aquatic Animals.International journal of molecular sciences · 2024Article
- Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum.Molecular biology reports · 2024Article
- The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis ofInternational journal of molecular sciences · 2023Article
- Optimization of Sperm Cryopreservation Formulation inInternational journal of molecular sciences · 2023Article
- Molecular Cloning of Dynein Heavy Chain and the Effect of Dynein Inhibition on the Testicular Function ofAnimals : an open access journal from MDPI · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The mechanism of acrosome formation in the crab sperm is a hot topic in crustacean reproduction research. Dynein is a motor protein that performs microtubule-dependent retrograde transport and plays an essential role in spermatogenesis. However, whether cytoplasmic dynein participates in acrosome formation in the crab sperm remains poorly understood. In this study, we cloned the cytoplasmic dynein intermediate chain gene (Pt-DIC) from Portunus trituberculatus testis. Pt-DIC is composed of a p150glued-binding domain, a dynein light chain (DLC)-binding domain, and a dynein heavy chain (DHC)-binding domain. The Pt-DIC gene is widely expressed in different tissues, showing the highest expression in the testis, and it is expressed in different stages of spermatid development, indicating important functions in spermatogenesis. We further observed the colocalization of Pt-DIC and Pt-DHC, Pt-DHC and tubulin, and Pt-DHC and GM130, and the results indicated that cytoplasmic dynein may participate in nuclear shaping and acrosome formation via vesicle transport. In addition, we examined the colocalization of Pt-DHC and a mitochondrion (MT) tracker and that of Pt-DHC and prohibitin (PHB). The results indicated that cytoplasmic dynein participated in mitochondrial transport and mitochondrial degradation. Taken together, these results support the hypothesis that cytoplasmic dynein participates in acrosome formation, nuclear shaping, and mitochondrial transport during spermiogenesis in P. trituberculatus. This study will provide valuable guidance for the artificial fertilization and reproduction of P. trituberculatus.
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