Evidence map›Paper›PMID 34475414›Full record

ArticleScientific data2021

Cydrasil 3, a curated 16S rRNA gene reference package and web app for cyanobacterial phylogenetic placement.

Daniel Roush, Ana Giraldo-Silva, Ferran Garcia-Pichel

Open access · goldAbstract readDataset
In one paragraph

Article in Scientific data, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 43 citations in OpenAlex.

  1. Assessing the implementation of crustivoltaics inApplied and environmental microbiology · 2026
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  12. Microbiome analysis revealsApplied and environmental microbiology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Daniel RoushSchool of Life Sciences, Arizona State University, 85282, Tempe, Arizona, USA.ORCID 0000-0001-8025-2117
Ana Giraldo-SilvaSchool of Life Sciences, Arizona State University, 85282, Tempe, Arizona, USA.
Ferran Garcia-PichelSchool of Life Sciences, Arizona State University, 85282, Tempe, Arizona, USA. ferran@asu.edu.ORCID 0000-0003-1383-1981
Arizona State University · US

Funding

National Science Foundation (NSF) 1224939National Science Foundation (NSF) 1449501
6 · The paper itself

Abstract

Cyanobacteria are a widespread and important bacterial phylum, responsible for a significant portion of global carbon and nitrogen fixation. Unfortunately, reliable and accurate automated classification of cyanobacterial 16S rRNA gene sequences is muddled by conflicting systematic frameworks, inconsistent taxonomic definitions (including the phylum itself), and database errors. To address this, we introduce Cydrasil 3 ( https://www.cydrasil.org ), a curated 16S rRNA gene reference package, database, and web application designed to provide a full phylogenetic perspective for cyanobacterial systematics and routine identification. Cydrasil 3 contains over 1300 manually curated sequences longer than 1100 base pairs and can be used for phylogenetic placement or as a reference sequence set for de novo phylogenetic reconstructions. The web application (utilizing PaPaRA and EPA-ng) can place thousands of sequences into the reference tree and has detailed instructions on how to analyze results. While the Cydrasil web application offers no taxonomic assignments, it instead provides phylogenetic placement, as well as a searchable database with curation notes and metadata, and a mechanism for community feedback.

Indexed as

Mobile ApplicationsPhylogenyCyanobacteriaDatabases, GeneticGenes, rRNAInternetRNA, Ribosomal, 16SRNA, Ribosomal, 16S

Identifiers

PMID34475414
PMCPMC8413452
OpenAlexW3197770201

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.