Evidence map›Paper›PMID 34475211›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2021

Biochemical reconstitution defines new functions for membrane-bound glycosidases in assembly of the bacterial cell wall.

Atsushi Taguchi, Julia E Page, Ho-Ching Tiffany Tsui, Malcolm E Winkler, Suzanne Walker

Open access · greenAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 39 citations in OpenAlex.

  1. Structural Insights into MltC fromJournal of microbiology and biotechnology · 2026
    Article
  2. Article
  3. Inactivation of KhpB (EloR/Jag) inApplied and environmental microbiology · 2025
    Article
  4. Peptidoglycan polymerase function and regulation.Microbiology and molecular biology reviews : MMBR · 2025
    Review
  5. Article
  6. Article
  7. SpbR controls lipoteichoic acid length by directly inhibiting signal peptidase SpsB inProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  8. A novelmSphere · 2025
    Article
  9. Article
  10. Article
  11. Article
  12. Elongasome core proteins and class A PBP1a display zonal, processive movement at the midcell ofProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  13. Chemical science · 2024
    Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Metal cofactor stabilization by a partner protein is a widespread strategy employed for amidase activation.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Atsushi TaguchiDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.ORCID 0000-0003-4962-0393
Julia E PageDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.ORCID 0000-0002-9884-2382
Ho-Ching Tiffany TsuiDepartment of Biology, Indiana University Bloomington, Bloomington, IN 47405.ORCID 0000-0003-0849-874X
Malcolm E WinklerDepartment of Biology, Indiana University Bloomington, Bloomington, IN 47405.ORCID 0000-0002-1482-2588
Suzanne WalkerDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115; suzanne_walker@hms.harvard.edu.ORCID 0000-0002-0545-914X
Harvard University · USIndiana University Bloomington · US

Funding

Medical Scientist Training ProgramT32GM007753 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI WALENSKY, LOREN DAVID · 1985 to 2021
$50.0M
Discovery and characterization of new bacterial cell wall targets and inhibitors to treat resistant infectionsR01AI148752 · NIAID · HARVARD MEDICAL SCHOOL · PI Daniel Kahne, Suzanne Walker · 2020 to 2026
$5.5M
New Regulatory Interactions and Circuits that Mediate the Dynamics, Homeostasis, and Stress Responses of Peptidoglycan Synthesis in the Superbug Streptococcus pneumoniaeR35GM131767 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI MALCOLM E. WINKLER · 2019 to 2026
$4.8M
Release of Extracellular DNA during Biofilm Formation in Staphylococcus aureusR01AI139011 · NIAID · HARVARD UNIVERSITY · PI KAHNE, DANIEL, LOSICK, RICHARD MARC · 2018 to 2022
$3.2M
Understanding the Regulation and Biological Roles of Peptidoglycan Hydrolases in Staphylococcus aureusF30AI156972 · NIAID · HARVARD MEDICAL SCHOOL · PI PAGE, JULIA ELAINE · 2021 to 2023
$142k
NIAID NIH HHS F30 AI156972NIAID NIH HHS R01 AI139011NIAID NIH HHS R01 AI148752NIGMS NIH HHS R35 GM131767NIGMS NIH HHS T32 GM007753
6 · The paper itself

Abstract

The peptidoglycan cell wall is a macromolecular structure that encases bacteria and is essential for their survival. Proper assembly of the cell wall requires peptidoglycan synthases as well as membrane-bound cleavage enzymes that control where new peptidoglycan is made and inserted. Previous studies have shown that two membrane-bound proteins in

Indexed as

Amino Acid SubstitutionBacterial ProteinsCarbohydrate SequenceCell WallGlycoside HydrolasesHydrolysisPeptidoglycanStreptococcus pneumoniaeBacterial ProteinsGlycoside HydrolasesPeptidoglycanglycosidaselytic transglycosylasemuramidasepeptidoglycan

Identifiers

PMID34475211
PMCPMC8433521
OpenAlexW3133710833

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.