ArticlePloS one2021
Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Melatonin Supplementation Increases Oocyte Recovery and Quality Associated with Altered Follicular Steroidogenesis and Redox Status in Dairy Cows.Antioxidants (Basel, Switzerland) · 2026Article
- In vivo use of melatonin improves oocyte developmental competence and blastocyst development rate in indigenous donor cow during summer.Tropical animal health and production · 2026Article
- The Impact of Melatonin on Cellular Dynamics and Gene Expression of Bovine Embryos Cultured Under Low and High Oxygen Tension.Molecular reproduction and development · 2026Article
- Melatonin Supplementation in Sex-Sorted Nili-Ravi Buffalo Semen: Effect on Sperm Quality, Subsequent in Vitro Embryo Development, and Pregnancy Outcomes.Antioxidants (Basel, Switzerland) · 2026Article
- Metabolomic Markers Reveal How hCG-Ketoprofen Intervention Increase Pregnancy Percentage Following Timed Artificial Insemination in Dairy Cows.Animals : an open access journal from MDPI · 2026Article
- Melatonin: A Silver Bullet for Fertility and Reproductive Health.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Melatonin During Pre-Maturation and Its Effects on Bovine Oocyte Competence.Antioxidants (Basel, Switzerland) · 2025Article
- Galangin Regulates Oxidative Stress Levels in Porcine Embryos Through Interaction with the Neh1 Domain of Nrf2.Antioxidants (Basel, Switzerland) · 2025Article
- The effect of melatonin implants on the embryo yield and oxidative stress levels in superovulated Holstein heifers.Veterinary research communications · 2025Article
- Factors Affecting the Success of Ovum Pick-Up, In Vitro Production and Cryopreservation of Embryos in Cattle.Animals : an open access journal from MDPI · 2025Review
- Effect of Melatonin Supplementation on In Vitro Developmental Competence of Bovine Oocyte: A Systematic Review and Meta-Analysis.Veterinary medicine international · 2025Review
- Melatonin decreases excessive polyspermy for single oocyte in pigs through the MT2 receptor.Scientific reports · 2024Article
- Comparative developmental competence ofVeterinary world · 2024Article
- Melatonin in animal husbandry: functions and applications.Frontiers in veterinary science · 2024Review
- Melatonin Protects Bovine Spermatozoa by Reinforcing Their Antioxidant Defenses.Animals : an open access journal from MDPI · 2023Article
- Assessment of developmental rate of mouse embryos yielded from in vitro fertilization of the oocyte with treatment of melatonin and vitamin C simultaneously.BMC women's health · 2023Article
- Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes.Frontiers in endocrinology · 2023Article
- Coping with Oxidative Stress in Reproductive Pathophysiology and Assisted Reproduction: Melatonin as an Emerging Therapeutical Tool.Antioxidants (Basel, Switzerland) · 2022Review
- Application of Nanoparticles and Melatonin for Cryopreservation of Gametes and Embryos.Current issues in molecular biology · 2022Review
- Exogenous Melatonin in the Culture Medium Does Not Affect the Development of In Vivo-Derived Pig Embryos but Substantially Improves the Quality of In Vitro-Produced Embryos.Antioxidants (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The developmental competence of male and female gametes is frequently reduced under in vitro conditions, mainly due to oxidative stress during handling. The amino-acid derived hormone melatonin has emerged as a potent non-enzymatic antioxidant in many biological systems. The goal of the present study was to evaluate the effects of melatonin on post-thaw sperm quality, fertilizing ability, and embryo development and competence in vitro after in vitro fertilization. Frozen-thawed bovine spermatozoa were incubated either in the presence of 10-11 M melatonin (MT), or its solvent (ethanol; Sham-Control), or plain Tyrode's Albumin Lactate Pyruvate medium (TALP, Control). Computer-Assisted Sperm Analysis (CASA) and flow cytometry data after 30 min, 120 min, and 180 min incubation did not reveal any significant effects of melatonin on average motility parameters, sperm subpopulation structure as determined by hierarchical cluster, or on the percentage of viable, acrosome intact sperm, or viable sperm with active mitochondria. Nevertheless, in vitro matured cumulus-oocyte-complexes fertilized with spermatozoa which had been preincubated with 10-11 M melatonin (MT-Sperm) showed higher (P < 0.01) rates of monospermic fertilization, reduced (P < 0.05) polyspermy and enhanced (P < 0.05) embryo development compared to the Control group. Moreover, the relative abundance of MAPK13 in the in vitro-derived blastocysts was greater (P < 0.05) than observed in the Control group. In conclusion, adding melatonin to the sperm-preparation protocol for bovine IVF improved proper fertilization and enhanced embryonic development and competence in vitro.
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