Evidence map›Paper›PMID 34473104›Full record

ArticleActa crystallographica. Section F, Structural biology communications2021

Structural characterization of hexameric shell proteins from two types of choline-utilization bacterial microcompartments.

Jessica M Ochoa, Oscar Mijares, Andrea A Acosta, Xavier Escoto, Nancy Leon-Rivera, Joanna D Marshall, Michael R Sawaya, Todd O Yeates

Abstract read
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Article in Acta crystallographica. Section F, Structural biology communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Architectural and biochemical advances in understanding microbial COThe Plant journal : for cell and molecular biology · 2026
    Review
  2. Article
  3. Article
  4. Ethanolamine-induced assembly of microcompartments is required forbioRxiv : the preprint server for biology · 2024
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jessica M OchoaUCLA Molecular Biology Institute, University of California Los Angeles, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA.ORCID 0000-0003-2389-0870
Oscar MijaresWhittier College, 13406 East Philadelphia Street, Whittier, CA 90602, USA.
Andrea A AcostaWhittier College, 13406 East Philadelphia Street, Whittier, CA 90602, USA.ORCID 0000-0002-2393-6341
Xavier EscotoDepartment of Chemistry and Biochemistry, University of California Los Angeles, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA.
Nancy Leon-RiveraWhittier College, 13406 East Philadelphia Street, Whittier, CA 90602, USA.ORCID 0000-0003-0231-492X
Joanna D MarshallDepartment of Chemistry and Biochemistry, University of California Los Angeles, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA.
Michael R SawayaUCLA-DOE Institute of Genomics and Proteomics, University of California Los Angeles, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA.ORCID 0000-0003-0874-9043
Todd O YeatesUCLA Molecular Biology Institute, University of California Los Angeles, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA.ORCID 0000-0001-5709-9839

Funding

User Training and OutreachP30GM124165 · NIGMS · CORNELL UNIVERSITY · PI Nozomi Ando, J Christopher Fromme · 2018 to 2026
$34.2M
Dissecting the structure, function, and mechanisms of diverse protein-based metabolic organelles in bacteriaR01AI081146 · NIAID · IOWA STATE UNIVERSITY · PI BOBIK, THOMAS AQUINAS, YEATES, TODD O · 2009 to 2022
$6.5M
Pixel Array Detector for Macromolecular CrystallographyS10OD021527 · OD · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2016 to 2016
$2.0M
National Institutes of Health, National Institute of Allergy and Infectious Diseases R01AI081146NIAID NIH HHS R01 AI081146NIGMS NIH HHS P30 GM124165NIH HHS S10 OD021527U.S. Department of Energy, Office of Science DE-FC02-02ER63421
6 · The paper itself

Abstract

Bacterial microcompartments are large supramolecular structures comprising an outer proteinaceous shell that encapsulates various enzymes in order to optimize metabolic processes. The outer shells of bacterial microcompartments are made of several thousand protein subunits, generally forming hexameric building blocks based on the canonical bacterial microcompartment (BMC) domain. Among the diverse metabolic types of bacterial microcompartments, the structures of those that use glycyl radical enzymes to metabolize choline have not been adequately characterized. Here, six structures of hexameric shell proteins from type I and type II choline-utilization microcompartments are reported. Sequence and structure analysis reveals electrostatic surface properties that are shared between the four types of shell proteins described here.

Indexed as

Amino Acid SequenceBacterial ProteinsCholineCrystallography, X-RayEscherichia coliOrganellesProtein ConformationSequence HomologyStreptococcus intermediusBacterial ProteinsCholinebacterial microcompartmentsbacterial organellesBMCcholineglycyl radicalsshell proteins

Identifiers

PMID34473104
PMCPMC8411931

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.