Evidence map›Paper›PMID 34471908›Full record

ArticleSTAR protocols2021

Sequential staining of HIV gp140 to capture antigen-specific human B cells via flow cytometry.

Samantha M Townsley, David J Leggat, Madhu Prabhakaran, Adrian B McDermott, Shelly J Krebs

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Samantha M TownsleyU.S. Military HIV Research Program, Center of Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
David J LeggatVaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA.
Madhu PrabhakaranVaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA.
Adrian B McDermottVaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA.
Shelly J KrebsU.S. Military HIV Research Program, Center of Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
National Institutes of Health · USHenry M. Jackson Foundation · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protocols for efficient capture of antigen-specific B cells (ASBCs) are useful for understanding pathogen-specific B-cell responses during natural infection or vaccination. Fluorescently labeled tetramerized probes are classically used to capture ASBCs, but many occlude valuable epitopes available for B-cell receptor binding. Here, we describe a bead assay to confirm ASBC receptor accessibility on probes and a sequential staining process to capture HIV gp140-specific B cells from human peripheral blood mononuclear cells. For complete details on the use and execution of this protocol, please refer to Townsley et al. (2021).

Indexed as

env Gene Products, Human Immunodeficiency VirusB-LymphocytesFlow CytometryHumansLeukocytes, MononuclearMolecular ProbesReceptors, Antigen, B-CellStaining and Labelingenv Gene Products, Human Immunodeficiency Virusgp140 envelope protein, Human immunodeficiency virus 1Molecular ProbesReceptors, Antigen, B-CellFlow Cytometry/Mass CytometryImmunologyMolecular/Chemical Probes

Identifiers

PMID34471908
PMCPMC8390768
OpenAlexW3193801762

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.