ArticleiScience2021
Mailing viable mouse freeze-dried spermatozoa on postcards.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Monosodium glutamate protects DNA of freeze-dried somatic cells and improves development of somatic cell nuclear transfer embryos.The Journal of reproduction and development · 2026Article
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Improved birth rates via rehydration of mouse freeze-dried spermatozoa using high-temperature ultrapure water.PloS one · 2026Article
- Research progress on novel bionic cryoprotectants in gamete and embryo cryopreservation.Frontiers in veterinary science · 2026Review
- Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage.Scientific reports · 2025Article
- Can Humanity Thrive Beyond the Galaxy?The Journal of reproduction and development · 2025Review
- Method for long-term room temperature storage of mouse freeze-dried sperm.Scientific reports · 2025Article
- Production of Healthy Mice from Freeze-Dried Spermatozoa Preserved at Room Temperature.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A novel, simplified method to prepare and preserve freeze-dried mouse sperm in plastic microtubes.The Journal of reproduction and development · 2023Article
- Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage.Frontiers in veterinary science · 2023Article
- Production of mouse offspring from zygotes fertilized with freeze-dried spermatids.Scientific reports · 2022Article
- Production of offspring from vacuum-dried mouse spermatozoa and assessing the effect of drying conditions on sperm DNA and embryo development.The Journal of reproduction and development · 2022Article
- Long-term preservation of germ cells and gonadal tissues at ambient temperatures.Reproduction & fertility · 2022Review
- Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?Journal of assisted reproduction and genetics · 2022Article
- Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets.STAR protocols · 2021Article
- Hyperosmotic HEPES-CZB Enhances Post-ICSI Survival of C57BL/6N Mouse Oocytes.Reproductive medicine and biologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Freeze-drying techniques allow the preservation of mammalian spermatozoa without using liquid nitrogen. However, the current method requires the use of glass ampoules, which are breakable, expensive, and bulky to store or transport. In this study, we evaluated whether mouse freeze-dried (FD) spermatozoa can be preserved and transported on thin materials. In this study, we demonstrated that FD sperm can be preserved in thin plastic sheets. Its DNA integrity was comparable to that of glass ampoule spermatozoa, and healthy offspring were obtained after preservation at -30°C for more than 3 months. We attached preserved FD sperm to postcards, and transported these to other laboratory inexpensively at room temperatures without any protection. This method will facilitate the preservation of thousands of mouse strains in a single card holder, promote collaboration between laboratories, conservation of genetic resources, and assisted reproductive technology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.