Evidence map›Paper›PMID 34471685›Full record

ArticleACS central science2021

Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells.

Jonathan Tyson, Kevin Hu, Shuai Zheng, Phylicia Kidd, Neville Dadina, Ling Chu, Derek Toomre, Joerg Bewersdorf, Alanna Schepartz

Abstract read
In one paragraph

Article in ACS central science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2023
    Article
  20. Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (International ed. in English) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jonathan TysonDepartment of Chemistry, University of California, Berkeley, California 94720, United States.
Kevin HuDepartment of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Shuai ZhengDepartment of Chemistry, University of California, Berkeley, California 94720, United States.
Phylicia KiddDepartment of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Neville DadinaDepartment of Chemistry, University of California, Berkeley, California 94720, United States.
Ling ChuDepartment of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Derek ToomreDepartment of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Joerg BewersdorfDepartment of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Alanna SchepartzDepartment of Chemistry, University of California, Berkeley, California 94720, United States.ORCID https://orcid.org/0000-0003-2127-3932

Funding

Yale Diabetes Research CenterP30DK045735 · NIDDK · YALE UNIVERSITY · PI Richard A Flavell · 1993 to 2026
$44.0M
Fluorescence tools that illuminate biology and inspire translationR35GM134963 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Alanna Schepartz · 2020 to 2026
$4.7M
Enabling Nanoscale Dynamic Imaging of Vesicles and OrganellesR01GM118486 · NIGMS · YALE UNIVERSITY · PI BADDELEY, DAVID, BEWERSDORF, JOERG · 2016 to 2019
$3.4M
Convergent Graduate Training in Engineering, Physics and BiologyT32EB019941 · NIBIB · YALE UNIVERSITY · PI O'HERN, COREY · 2016 to 2020
$998k
Expanding the HIDE nanoscopy toolbox: More organelles, colors, and modalitiesR01GM131372 · NIGMS · YALE UNIVERSITY · PI SCHEPARTZ, ALANNA · 2019 to 2019
$299k
NIBIB NIH HHS T32 EB019941NIDDK NIH HHS P30 DK045735NIGMS NIH HHS R01 GM118486NIGMS NIH HHS R01 GM131372NIGMS NIH HHS R35 GM134963Wellcome Trust 203285/Z/16/Z
6 · The paper itself

Abstract

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale

Identifiers

PMID34471685
PMCPMC8393207

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.