Evidence map›Paper›PMID 34470609›Full record

ArticleMolecular medicine (Cambridge, Mass.)2021

The long non-coding RNA DKFZp434J0226 regulates the alternative splicing process through phosphorylation of SF3B6 in PDAC.

Jinglei Li, Hanxing Tong, Dongping Li, Qiuyu Jiang, Yong Zhang, Wenqing Tang, Dayong Jin, She Chen, Xinyu Qin, Si Zhang and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine (Cambridge, Mass.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Jinglei Li *Department of General Surgery, Zhongshan Hospital, Fudan University, 180 FengLin Road, Shanghai, 200032, China.
Hanxing Tong *Department of General Surgery, Zhongshan Hospital, Fudan University, 180 FengLin Road, Shanghai, 200032, China.
Dongping Li *Department of Gastroenterology and Hepatology, Zhongshan Hospital, Shanghai Institute of Liver Disease, Fudan University, 180 FengLin Road, Shanghai, 200032, China.
Qiuyu JiangDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Shanghai Institute of Liver Disease, Fudan University, 180 FengLin Road, Shanghai, 200032, China.
Yong ZhangDepartment of General Surgery, Zhongshan Hospital, Fudan University, 180 FengLin Road, Shanghai, 200032, China.
Wenqing TangDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Shanghai Institute of Liver Disease, Fudan University, 180 FengLin Road, Shanghai, 200032, China.
Dayong JinDepartment of General Surgery, Zhongshan Hospital, Fudan University, 180 FengLin Road, Shanghai, 200032, China.
She ChenNHC Key Laboratory of Glycoconjugate Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, 130 DongAn Road, Shanghai, 200032, China.
Xinyu QinDepartment of General Surgery, Zhongshan Hospital, Fudan University, 180 FengLin Road, Shanghai, 200032, China. qin.xinyu@zs-hospital.sh.cn.
Si ZhangNHC Key Laboratory of Glycoconjugate Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, 130 DongAn Road, Shanghai, 200032, China. zhangsi@fudan.edu.cn.ORCID 0000-0002-5682-4995
Ruyi XueDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Shanghai Institute of Liver Disease, Fudan University, 180 FengLin Road, Shanghai, 200032, China. xue.ruyi@zs-hospital.sh.cn.
Fudan University · CNShanghai Medical College of Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLong noncoding RNAs (lncRNAs), a type of pervasive genes that regulates various biological processes, are differentially expressed in different types of malignant tumors. The role of lncRNAs in the carcinogenesis of pancreatic ductal adenocarcinoma (PDAC) remains unclear. Here, we investigated the role of the lncRNA DKFZp434J0226 in PDAC.

methodsAberrantly expressed mRNAs and lncRNAs among six PDAC and paired non-tumorous tissues were profiled using microarray analysis. Quantitative real-time polymerase chain reaction was used to evaluate DKFZp434J0226 expression in PDAC tissues. CCK-8 assay, wound-healing assay, soft agar colony formation assay, and transwell assay were performed to assess the invasiveness and proliferation of PDAC cells. Furthermore, RNA pull-down, immunofluorescence, RNA immunoprecipitation, and western blotting assays were performed to investigate the association between DKFZp434J0226 and SF3B6. Tumor xenografts in mice were used to test for tumor formation in vivo.

resultsIn our study, 222 mRNAs and 128 lncRNAs were aberrantly expressed (≥ twofold change). Of these, 66 mRNAs and 53 lncRNAs were upregulated, while 75 lncRNAs and 156 mRNAs were downregulated. KEGG pathway analysis and the Gene ontology category indicated that these genes were associated with the regulation of mRNA alternative splicing and metabolic balance. Clinical analyses revealed that overexpression of DKFZp434J0226 was associated with worse tumor grading, frequent perineural invasion, advanced tumor-node-metastasis stage, and decreased overall survival and time to progression. Functional assays demonstrated that DKFZp434J0226 promoted PDAC cell migration, invasion, and growth in vitro and accelerated tumor proliferation in vivo. Mechanistically, DKFZp434J0226 interacted with the splicing factor SF3B6 and promoted its phosphorylation, which further regulated the alternative splicing of pre-mRNA.

conclusionsThis study indicates that DKFZp434J0226 regulates alternative splicing through phosphorylation of SF3B6 in PDAC and leads to an oncogenic phenotype in PDAC.

Indexed as

Alternative SplicingRNA, Long NoncodingAnimalsCarcinoma, Pancreatic DuctalCell Line, TumorCell MovementCell ProliferationComputational BiologyDisease Models, AnimalGene Expression ProfilingHumansMicePancreatic NeoplasmsPhosphorylationPrognosisProtein BindingRNA, Long NoncodingRNA Splicing FactorsDKFZp434J0226LncRNA expression signatureMicroarrayPancreatic ductal adenocarcinoma

Identifiers

PMID34470609
PMCPMC8411526
OpenAlexW3198241807

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.