ArticleCell systems2021
Assembling stable syntrophic Escherichia coli communities by comprehensively identifying beneficiaries of secreted goods.
Article in Cell systems, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 40 citations in OpenAlex.
- Anaerobic riboflavin degradation by human gutJournal of bacteriology · 2026Article
- Article
- Burden-aware feedback control of microbial consortia.Nature communications · 2026Article
- Counting-based inference of mutant growth rates from pooled sequencing across growth regimes.bioRxiv : the preprint server for biology · 2026Article
- Anaerobic riboflavin degradation by human gutbioRxiv : the preprint server for biology · 2026Article
- Cobamide-based interactions between soil bacteria can be predicted based on monoculture growth.bioRxiv : the preprint server for biology · 2025Article
- Predicting drug inactivation by changes in bacterial growth dynamics.npj antimicrobials and resistance · 2025Article
- Microbial consortia for the conversion of biomass into fuels and chemicals.Nature communications · 2025Review
- Competitive fitness of Staphylococcus aureus against nasal commensals depends on biotin biosynthesis and acquisition.The ISME journal · 2025Article
- Asymmetric metabolic adaptations undermine stability in microbial syntrophy.ISME communications · 2025Article
- Article
- DyMMM-LEAPS: An ML-based framework for modulating evenness and stability in synthetic microbial communities.Biophysical journal · 2024Article
- Microbial life in slow and stopped lanes.Trends in microbiology · 2024Review
- Antibacterial activity of nonantibiotics is orthogonal to standard antibiotics.Science (New York, N.Y.) · 2024Article
- A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities.Nature microbiology · 2024Article
- Engineering E. coli strains using antibiotic-resistance-gene-free plasmids.Cell reports methods · 2023Article
- Mutualism reduces the severity of gene disruptions in predictable ways across microbial communities.The ISME journal · 2023Article
- Vitamin interdependencies predicted by metagenomics-informed network analyses and validated in microbial community microcosms.Nature communications · 2023Article
- Pesticide exposure and the microbiota-gut-brain axis.The ISME journal · 2023Review
- Spontaneously established syntrophic yeast communities improve bioproduction.Nature chemical biology · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Metabolic cross-feeding frequently underlies mutualistic relationships in natural microbial communities and is often exploited to assemble synthetic microbial consortia. We systematically identified all single-gene knockouts suitable for imposing cross-feeding in Escherichia coli and used this information to assemble syntrophic communities. Most strains benefiting from shared goods were dysfunctional in biosynthesis of amino acids, nucleotides, and vitamins or mutants in central carbon metabolism. We tested cross-feeding potency in 1,444 strain pairs and mapped the interaction network between all functional groups of mutants. This network revealed that auxotrophs for vitamins are optimal cooperators. Lastly, we monitored how assemblies composed of dozens of auxotrophs change over time and observed that they rapidly and repeatedly coalesced to seven strain consortia composed primarily from vitamin auxotrophs. The composition of emerging consortia suggests that they were stabilized by multiple cross-feeding interactions. We conclude that vitamins are ideal shared goods since they optimize consortium growth while still imposing member co-dependence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.