Evidence map›Paper›PMID 34469126›Full record

ArticleACS synthetic biology2021

Growth-Based, High-Throughput Selection for NADH Preference in an Oxygen-Dependent Biocatalyst.

Sarah Maxel, Samer Saleh, Edward King, Derek Aspacio, Linyue Zhang, Ray Luo, Han Li

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Sarah MaxelDepartments of Chemical and Biomolecular Engineering, University of California, Irvine, California 92697, United States.
Samer SalehDepartments of Chemical and Biomolecular Engineering, University of California, Irvine, California 92697, United States.
Edward KingMolecular Biology and Biochemistry, University of California, Irvine, California 92697, United States.
Derek AspacioDepartments of Chemical and Biomolecular Engineering, University of California, Irvine, California 92697, United States.
Linyue ZhangDepartments of Chemical and Biomolecular Engineering, University of California, Irvine, California 92697, United States.
Ray LuoDepartments of Chemical and Biomolecular Engineering, University of California, Irvine, California 92697, United States.ORCID 0000-0002-6346-8271
Han LiDepartments of Chemical and Biomolecular Engineering, University of California, Irvine, California 92697, United States.ORCID 0000-0002-6113-6433
University of California, Irvine · US

Funding

Engineering an unnatural redox cofactor (uRedox) system for efficient biosynthesis of medicinesDP2GM137427 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI LI, HAN · 2019 to 2022
$2.4M
NIGMS NIH HHS DP2 GM137427
6 · The paper itself

Abstract

Cyclohexanone monooxygenases (CHMO) consume molecular oxygen and NADPH to catalyze the valuable oxidation of cyclic ketones. However, CHMO usage is restricted by poor stability and stringent specificity for NADPH. Efforts to engineer CHMO have been limited by the sensitivity of the enzyme to perturbations in conformational dynamics and long-range interactions that cannot be predicted. We demonstrate an aerobic, high-throughput growth selection platform in

Indexed as

BiocatalysisDirected Molecular EvolutionEscherichia coliEscherichia coli ProteinsHigh-Throughput Screening AssaysKineticsMolecular Dynamics SimulationMutagenesis, Site-DirectedNADOxidation-ReductionOxygenOxygenasescyclohexanone oxygenaseEscherichia coli ProteinsNADOxygenOxygenasesBaeyer−Villiger monooxygenasecofactor specificitycyclohexanone monooxygenasedirected evolutionhigh-throughput selectionredox balance

Identifiers

PMID34469126
PMCPMC10362907
OpenAlexW3198419527

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.