Virulence and intermediate resistance to high-end antibiotic (teicoplanin) among coagulase-negative staphylococci sourced from retail market fish.

K H Muneeb, S Sudha, G K Sivaraman, Bibek Shome, Jennifer Cole, Mark Holmes

Abstract read

In one paragraph

Article in Archives of microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.3field-weighted citation impact, top 39% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

Trends of Antimicrobial Susceptibility in Clinically Significant Coagulase-Negative Staphylococci Isolated from Cerebrospinal Fluid Cultures in Neurosurgical Adults: a Nine-Year Analysis.Microbiology spectrum · 2022
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

K H MuneebMicrobiology Fermentation and Biotechnology Division, ICAR-Central Institute of Fisheries Technology, Matsyapuri P. O, Willingdon Island, Kochi, Kerala, 682 029, India.ORCID http://orcid.org/0000-0003-4747-9162

S SudhaMicrobiology Fermentation and Biotechnology Division, ICAR-Central Institute of Fisheries Technology, Matsyapuri P. O, Willingdon Island, Kochi, Kerala, 682 029, India.ORCID http://orcid.org/0000-0001-5123-7313

G K SivaramanMicrobiology Fermentation and Biotechnology Division, ICAR-Central Institute of Fisheries Technology, Matsyapuri P. O, Willingdon Island, Kochi, Kerala, 682 029, India. gkshivraman@gmail.com.ORCID http://orcid.org/0000-0003-0150-7295

Bibek ShomeDepartment of Disease Investigation, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bangalore, India.

Jennifer ColeDepartment of Geography, Royal Holloway, University of London, London, UK.ORCID http://orcid.org/0000-0001-8787-8892

Mark HolmesDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.

Central Institute of Fisheries Technology · INNational Institute Of Veterinary Epidemiology And Disease Informatics · INRoyal Holloway University of London · GBUniversity of Cambridge · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study reports the distribution of enterotoxigenic determinants among staphylococci and the susceptibility of staphylococci to various classes of antibiotics. We observed all the isolates as resistant to beta-lactam antibiotics and a few as resistant to non-beta-lactam antibiotics such as clindamycin (47.4%), erythromycin (44.7%), gentamicin (23.7%), norfloxacin (34.2%), tetracycline (26.3%), trimethoprim-sulfamethoxazole (15.8%) etc. The resistance of S. sciuri (n = 1) and S. haemolyticus (n = 1) to rifampicin and intermediate resistance of S. gallinarum (n = 2) to teicoplanin, a high-end antibiotic, are also observed in this study. The multidrug-resistance (≥ 3 classes of antibiotics) was recorded in 23 (60.5%) isolates. The virulomes such as sea, seb, seg and sei were identified predominantly in S. haemolyticus. Surprisingly, certain isolates which were phenotypically confirmed as biofilm-producers by Congo red agar (CRA) test did not harbor biofilm-associated loci. This implies the protein-mediated mechanism of biofilm formation as an alternative to polysaccharide intercellular adhesin (PIA) in staphylococci. However, icaAD locus which encodes PIA was identified in 10 (26.3%) isolates and the eno locus, encoding elastin-binding protein which can accelerate the biofilm production, is identified in all the isolates. The possession of type V SCCmec elements by the S. haemolyticus (15.8%) raised the concern about the rapid dissemination of mecA gene to other species of staphylococci including the virulent S. aureus. In short, this study acknowledges the toxigenicity of coagulase-negative staphylococci (CoNS). Through this study, surveillance of antimicrobial resistance and transference of virulomes in staphylococci is warranted.

Indexed as

Anti-Bacterial AgentsStaphylococcal InfectionsAnimalsCoagulaseMicrobial Sensitivity TestsStaphylococcus aureusTeicoplaninVirulenceAnti-Bacterial AgentsCoagulaseTeicoplaninCoagulase-negative staphylococciEnterotoxinsmecA geneType V SCCmec elementsVirulence genes

Identifiers

PMID34468806

DOI10.1007/978-1-4615-2181-5_11

OpenAlexW3197290360

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.