ArticleeLife2021
In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Bacterial nanotubes extend from a tubeosome organelle to establish an intercellular bridge.bioRxiv : the preprint server for biology · 2026Article
- Morphological plasticity of endophytic Chitinophaga pinensis.Antonie van Leeuwenhoek · 2026Article
- Ultrastructure Analysis by Cryo-Electron Tomography Revealed Mesosomes in the Gram-negative Delftia Acidovorans.Microbial ecology · 2026Article
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- In situ NMR reveals a pH sensor motif in an outer membrane protein that drives bacterial vesicle production.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Outer membrane tube formation bymBio · 2025Article
- The bacterial type three secretion system induces mechanoporation of vacuolar membranes.PLoS biology · 2025Article
- From genes to nanotubes: exploring the UV-resistome in the Andean extremophile Exiguobacterium sp. S17.Extremophiles : life under extreme conditions · 2025Article
- Standardizing experimental approaches to investigate interactions between bacteria and ectomycorrhizal fungi.FEMS microbiology reviews · 2025Review
- Horizontal gene transfer and beyond: the delivery of biological matter by bacterial membrane vesicles to host and bacterial cells.Current opinion in microbiology · 2024Review
- Capturing of extracellular vesicles derived from single cells ofLab on a chip · 2024Article
- An outer membrane vesicle specific lipoprotein promotesCurrent research in microbial sciences · 2024Article
- Characterization of membrane vesicles inmicroLife · 2023Article
- Recent advances in infectious disease research using cryo-electron tomography.Frontiers in molecular biosciences · 2023Review
- Identification of NpdA as the protein forming the surface layer inAccess microbiology · 2023Article
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- Outer Membrane Vesicles: Biogenesis, Functions, and Issues.Microbiology and molecular biology reviews : MMBR · 2022Review
- Discovery of a Novel Inner Membrane-Associated Bacterial Structure Related to the Flagellar Type III Secretion System.Journal of bacteriology · 2022Article
- Molecular architecture of bacterial type IV secretion systems.PLoS pathogens · 2022Review
Corrections and comments
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Authors and funding
18 authors at 10 institutions in 3 countries.
Funding
Abstract
The ability to produce outer membrane projections in the form of tubular membrane extensions (MEs) and membrane vesicles (MVs) is a widespread phenomenon among diderm bacteria. Despite this, our knowledge of the ultrastructure of these extensions and their associated protein complexes remains limited. Here, we surveyed the ultrastructure and formation of MEs and MVs, and their associated protein complexes, in tens of thousands of electron cryo-tomograms of ~90 bacterial species that we have collected for various projects over the past 15 years (Jensen lab database), in addition to data generated in the Briegel lab. We identified outer MEs and MVs in 13 diderm bacterial species and classified several major ultrastructures: (1) tubes with a uniform diameter (with or without an internal scaffold), (2) tubes with irregular diameter, (3) tubes with a vesicular dilation at their tip, (4) pearling tubes, (5) connected chains of vesicles (with or without neck-like connectors), (6) budding vesicles and nanopods. We also identified several protein complexes associated with these MEs and MVs which were distributed either randomly or exclusively at the tip. These complexes include a secretin-like structure and a novel crown-shaped structure observed primarily in vesicles from lysed cells. In total, this work helps to characterize the diversity of bacterial membrane projections and lays the groundwork for future research in this field.
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