ArticleJACS Au2021
Substrate Channeling by a Rationally Designed Fusion Protein in a Biocatalytic Cascade.
Article in JACS Au, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Antibiotic- and inducer-free biosynthesis of indican from crude glycerol using engineered probiotic Escherichia coli Nissle 1917.Bioresources and bioprocessing · 2026Article
- Tracing the origins of crmA megasynthase through lichen genomes.Current biology : CB · 2026Article
- Complete enzyme clustering enhances coenzyme Q biosynthesis via substrate channeling.Nature communications · 2026Article
- Aqueous-Phase Polycondensation of Hydroxy Fatty Acids via a Whole-Cell CoA Activation-Acyltransferase Cascade.JACS Au · 2026Article
- Construction of artificial organellesChemical science · 2025Review
- Structural insights into a bacterial terpene cyclase fused with haloacid Dehalogenase-like phosphatase.Chemical science · 2025Article
- Activation-Free Upgrading of Carboxylic Acids to Aldehydes and Alcohols.bioRxiv : the preprint server for biology · 2025Article
- Leveraging EngineeredACS synthetic biology · 2025Article
- Development of Malate Biosensor-Containing Hydrogels and Living Cell-Based Sensors.International journal of molecular sciences · 2024Article
- Optimizing the conversion of phosphoenolpyruvate to lactate by enzymatic channeling with mixed nanoparticle display.Cell reports methods · 2024Article
- Advanced Electroanalysis for Electrosynthesis.ACS organic & inorganic Au · 2024Review
- Cellulose-coated emulsion micro-particles self-assemble with yeasts for cellulose bio-conversion.Scientific reports · 2024Article
- Plant-Produced Viral Nanoparticles as a Functionalized Catalytic Support for Metabolic Engineering.Plants (Basel, Switzerland) · 2024Article
- Structural Characterization and Molecular Dynamics Study of the REPI Fusion Protein fromBiomolecules · 2023Article
- Identification of vitamin BBiotechnology letters · 2023Article
- Self assembling nanoparticle enzyme clusters provide access to substrate channeling in multienzymatic cascades.Nature communications · 2023Article
- Engineering Catalytically Self-Sufficient P450s.Biochemistry · 2023Review
- Metal-Organic Enzyme Nanogels as Nanointegrated Self-Reporting Chemobiosensors.ACS applied materials & interfaces · 2022Article
- Combining a Genetically Engineered Oxidase with Hydrogen-Bonded Organic Frameworks (HOFs) for Highly Efficient Biocomposites.Angewandte Chemie (International ed. in English) · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Substrate channeling, where an intermediate in a multistep reaction is directed toward a reaction center rather than freely diffusing, offers several advantages when employed in catalytic cascades. Here we present a fusion enzyme comprised of an alcohol and aldehyde dehydrogenase, that is computationally designed to facilitate electrostatic substrate channeling using a cationic linker bridging the two structures. Rosetta protein folding software was utilized to determine an optimal linker placement, added to the truncated termini of the proteins, which is as close as possible to the active sites of the enzymes without disrupting critical catalytic residues. With improvements in stability, product selectivity (90%), and catalyst turnover frequency, representing 500-fold increased activity compared to the unbound enzymes and nearly 140-fold for a neutral-linked fusion enzyme, this design strategy holds promise for making other multistep catalytic processes more sustainable and efficient.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.