Evidence map›Paper›PMID 34467227›Full record

ArticleSTAR protocols2021

Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag.

Yuefeng Li, Kiran Nakka, Thomas Olender, Philippe Gingras-Gelinas, Matthew Man-Kin Wong, Daniel C L Robinson, Hina Bandukwala, Carmen G Palii, Odile Neyret, Marjorie Brand and 2 more

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Yuefeng LiSprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
Kiran NakkaSprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
Thomas OlenderSprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
Philippe Gingras-GelinasMolecular Biology Platform, Institut de Recherche Clinique de Montreal, Montreal, QC, Canada.
Matthew Man-Kin WongSprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
Daniel C L RobinsonSprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
Hina BandukwalaSprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
Carmen G PaliiSprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
Odile NeyretMolecular Biology Platform, Institut de Recherche Clinique de Montreal, Montreal, QC, Canada.
Marjorie BrandSprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
Alexandre BlaisOttawa Institute for Systems Biology, University of Ottawa, Ottawa, ON, Canada.
F Jeffrey DilworthSprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
University of Ottawa · CAMontreal Clinical Research Institute · CAOttawa Hospital · CA

Funding

CIHR 201708IGHCIHR FDN-143330CIHR MOP119458
6 · The paper itself

Abstract

Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50,000 activated MuSCs isolated from injured mouse hindlimb muscles. We have applied this same approach to human CD34

Indexed as

AnimalsCardiotoxinsChromatinHematopoietic Stem CellsHistonesHumansMiceMice, TransgenicMolecular BiologyMuscle, SkeletalPolymerase Chain ReactionStem CellsTranscription FactorsCardiotoxinsChromatinHistonesTranscription Factorscell biologycell isolationChIPseqchromatin immunoprecipitation (ChIP)flow cytometry/mass cytometrymolecular biologysequence analysissequencingstem cells

Identifiers

PMID34467227
PMCPMC8384913
OpenAlexW3194951089

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.