Evidence map›Paper›PMID 34467222›Full record

ArticleSTAR protocols2021

Application of pseudotyped virus particles to monitor Ebola virus and SARS-CoV-2 viral entry in human cell lines.

Madeleine Eichler, Ebru Aksi, Josef Pfeilschifter, Gergely Imre

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Madeleine EichlerInstitute of General Pharmacology and Toxicology, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany.
Ebru AksiInstitute of General Pharmacology and Toxicology, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany.
Josef PfeilschifterInstitute of General Pharmacology and Toxicology, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany.
Gergely ImreInstitute of General Pharmacology and Toxicology, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany.
Goethe University Frankfurt · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Experimental work on highly pathogenic viruses such as Ebola virus (EBOV) and severe acute respiratory syndrome coronavirus-2 requires high-level biosafety facilities. Here, we provide a detailed step-by-step protocol which details the production and application of replication-incompetent murine leukemia virus-based pseudotyped particles to monitor and quantify the viral entry efficiency in human cell lines under biosafety level-2 conditions. We describe the use of viral particles encoding

Indexed as

Virus InternalizationCOVID-19EbolavirusHEK293 CellsHemorrhagic Fever, EbolaHumansSARS-CoV-2Viral PseudotypingVirionCell-based AssaysCell cultureImmunologyMicrobiologyMolecular Biology

Identifiers

PMID34467222
PMCPMC8390363
OpenAlexW3198238149

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.