ArticleGalen medical journal2019
Colonization of Mouse Spermatogonial Cells in Modified Soft Agar Culture System Utilizing Nanofibrous Scaffold: A New Approach.
Article in Galen medical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 9 citations in OpenAlex.
- A Comparative Analysis of Culture Systems with Human Amniotic Mesenchymal Stem Cells.Journal of reproduction & infertility · 2025Article
- In vivo and in vitro sperm production: an overview of the challenges and advances in male fertility restoration.Clinical and experimental reproductive medicine · 2024Article
- In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility.Cell and tissue research · 2023Review
- Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis.Stem cell research & therapy · 2023Review
- Advancing bovineFrontiers in bioengineering and biotechnology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSpermatogonial stem cells (SSCs) are considered in fertility management approaches of prepubertal boys facing cancer therapies. However, in vitro propagation has become an important issue due to a small number of SSCs in testicular tissue. The present study aimed to investigate a modified soft agar culture system by using a nanofibrous scaffold as a new approach to mimic in vivo conditions of SSCs development. MATERIALS AND
methodsThe SSCs were isolated from neonate mouse testes, cultured on polycaprolactone scaffold, and covered by a layer of soft agar for 2 weeks. Then, the number and diameter of colonies formed in experimental groups were measured and spermatogonial markers (i.e.,
resultsOur results indicated that the colonization rate of SSCs was significantly higher in the present modified soft agar culture system (P<0.05). Only
conclusionThe culture system by using nanofibrous scaffold and soft agar as a new culture method suggests the potential of this approach in SSCs enrichment and differentiation strategies for male infertility treatments, as well as in vitro spermatogenesis.
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Registered trials
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