ArticleBone research2021
3D assessment of intervertebral disc degeneration in zebrafish identifies changes in bone density that prime disc disease.
Article in Bone research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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Who cites it
44 citing papers in PubMed, 61 citations in OpenAlex.
- Gingerol-loaded hollow manganese dioxide nanoparticles attenuate intervertebral disc oxidative stress.RSC advances · 2026Article
- Harnessing piezoelectric stimulation to modulate PI3K-AKT signaling for intervertebral disc regeneration.Bioactive materials · 2026Article
- INHAND: Non-proliferative and proliferative lesions of fish - Chapter 12. Fish skeletal and tooth.Journal of toxicologic pathology · 2026Article
- DDRGK1 preserves intervertebral disc development through ufmylation.Cellular and molecular life sciences : CMLS · 2025Article
- The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity.Biology open · 2025Article
- What a pain in the back: etiology, diagnosis and future treatment directions for discogenic low back pain.Bone research · 2025Review
- Oxidative stress-induced intervertebral disc remodelling and elevated stiffness drive idiopathic scoliosis in preclinical models.Nature communications · 2025Article
- Combined antiresorptive and new anabolic drug approach in osteogenesis imperfecta zebrafish models.JBMR plus · 2025Article
- The importance of imperfect pre-clinical models in adolescent idiopathic scoliosis.Disease models & mechanisms · 2025Review
- Causal Relationship Between Cerebrospinal Fluid Metabolites and Intervertebral Disc Disease: A Bidirectional Mendelian Randomization Study.Diagnostics (Basel, Switzerland) · 2025Article
- Endplate remodeling: a key indicator of cigarette smoke exposure-induced intervertebral disc degeneration in a male rat model.JBMR plus · 2025Article
- MicroCT Scanning and Analysis of the Zebrafish Skeleton.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Exploring Causality Between Bone Mineral Density and Cervical Spondylosis: Bidirectional and Multivariable Mendelian Randomization Study.Journal of pain research · 2025Article
- Trends and emerging frontiers of mesenchymal stem cells in intervertebral disc degeneration: a bibliometric analysis (2000-2024).Frontiers in medicine · 2025Article
- Zebrafish Models for Skeletal and Extraskeletal Osteogenesis Imperfecta Features: Unveiling Pathophysiology and Paving the Way for Drug Discovery.Calcified tissue international · 2024Review
- Standardization of bone morphometry and mineral density assessments in zebrafish and other small laboratory fishes using X-ray radiography and micro-computed tomography.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024Article
- A multifunctional scaffold that promotes the scaffold-tissue interface integration and rescues the ROS microenvironment for repair of annulus fibrosus defects.Bioactive materials · 2024Article
- Tuning collective behaviour in zebrafish with genetic modification.PLoS computational biology · 2024Article
- Seeing the unseen: The role of bioimaging techniques for the diagnostic interventions in intervertebral disc degeneration.Bone reports · 2024Review
- Intervertebral Disk Degeneration and Bone Mineral Density: A Bidirectional Mendelian Randomization Study.Calcified tissue international · 2024Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 4 countries.
Funding
Abstract
Back pain is a common condition with a high social impact and represents a global health burden. Intervertebral disc disease (IVDD) is one of the major causes of back pain; no therapeutics are currently available to reverse this disease. The impact of bone mineral density (BMD) on IVDD has been controversial, with some studies suggesting osteoporosis as causative for IVDD and others suggesting it as protective for IVDD. Functional studies to evaluate the influence of genetic components of BMD in IVDD could highlight opportunities for drug development and repurposing. By taking a holistic 3D approach, we established an aging zebrafish model for spontaneous IVDD. Increased BMD in aging, detected by automated computational analysis, is caused by bone deformities at the endplates. However, aged zebrafish spines showed changes in bone morphology, microstructure, mineral heterogeneity, and increased fragility that resembled osteoporosis. Elements of the discs recapitulated IVDD symptoms found in humans: the intervertebral ligament (equivalent to the annulus fibrosus) showed disorganized collagen fibers and herniation, while the disc center (nucleus pulposus equivalent) showed dehydration and cellular abnormalities. We manipulated BMD in young zebrafish by mutating sp7 and cathepsin K, leading to low and high BMD, respectively. Remarkably, we detected IVDD in both groups, demonstrating that low BMD does not protect against IVDD, and we found a strong correlation between high BMD and IVDD. Deep learning was applied to high-resolution synchrotron µCT image data to analyze osteocyte 3D lacunar distribution and morphology, revealing a role of sp7 in controlling the osteocyte lacunar 3D profile. Our findings suggest potential avenues through which bone quality can be targeted to identify beneficial therapeutics for IVDD.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.