ArticlePoultry science2021
Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality?
Article in Poultry science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Mitochondria-targeted SkQ1 supplementation enhances post-thaw quality, cryosurvival, and functional competence of rooster spermatozoa.Poultry science · 2026Article
- An integrated anti-aging framework targeting NADRedox biology · 2026Review
- The effect of combined cryoprotectants on the cryotolerance of boar sperm.Animal bioscience · 2025Article
- Optimization of Duck Semen Freezing Procedure and Regulation of Oxidative Stress.Animals : an open access journal from MDPI · 2025Article
- Untargeted Metabolomic Identifies Potential Seasonal Biomarkers of Semen Quality in Duroc Boars.Biology · 2025Article
- Mitochondrial Regulation of Spermatozoa Function: Metabolism, Oxidative Stress and Therapeutic Insights.Animals : an open access journal from MDPI · 2025Review
- Which is more effective in thawing frozen rooster sperm: varying temperature or duration?Frontiers in veterinary science · 2025Article
- The effect of supplementing rosemary essential oil and selenium yeast in the diet on sperm parameters and antioxidant activity of semen in older broiler breeder roosters.Iranian journal of veterinary research · 2025Article
- Comparative assessment of thawing methods for frozen rooster sperm.Frontiers in veterinary science · 2025Article
- Effects of Supplementation of Different Antioxidants to Cryopreservation Extender on the Post-Thaw Quality of Rooster Semen-A Meta-Analysis.Animals : an open access journal from MDPI · 2024Article
- Effects of dietary basil (Veterinary and animal science · 2024Article
- Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function.Scientific reports · 2024Article
- Cryopreservation of Limited Sperm Using A Combination of Sucrose and Taurine, Loaded on Two Different Devices, and Thawed at Two Different Temperatures.International journal of fertility & sterility · 2024Article
- Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes.Animal reproduction · 2023Article
- Does Antioxidant Mitoquinone (MitoQ) Ameliorate Oxidative Stress in Frozen-Thawed Rooster Sperm?Animals : an open access journal from MDPI · 2022Article
- Effect of tempol and straw size on rooster sperm quality and fertility after post-thawing.Scientific reports · 2022Article
- Article
- Metabolomic analysis reveals spermatozoa and seminal plasma differences between Duroc and Liang guang Small-spotted pig.Frontiers in veterinary science · 2022Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study focuses on the effect of different levels of ergothioneine and thawing temperature on rooster semen cryopreservation. Semen was diluted in Lake extender containing ergothioneine at 5, 10, 15, and 20 µM and cryopreserved. Two thawing temperatures (37°C for 30 s and 60°C for 5 s) were consequently examined. Sperm motility parameter, membrane integrity, abnormal morphology, viability, apoptotic status, mitochondria activity, and lipid peroxidation were determined after freeze-thaw process. Ergothioneine levels of 5 and 10 µM led to higher (P < 0.05) total motility (66.58 ± 1.44 and 72.11±1.44, respectively) and average path velocity (VAP) (34.54 ± 0.89, 37.28 ± 0.89, respectively). Higher (P < 0.05) significant membrane integrity and mitochondria activity after freeze-thawing were observed in the groups supplemented with 10 µM ergothioneine (68.62 ± 1.24 and 69.12 ± 1.26, respectively). Also, 5 and 10 µM of ergothioneine led to the lowest significant percentage of apoptotic and dead sperm. The total motility and progressive motility resulted in significantly (P < 0.05) higher amount when sperm were thawed with 60°C (60.58 ± 0.91 and 24.76 ± 0.53, respectively) compared to thawed sperm in 37°C. The membrane integrity, viability and mitochondria activity led to significantly (P < 0.05) higher when sperm were thawed with 60°C (58.2 ± 0.78, 63.21 ± 0.80 and 56.85 ± 0.79, respectively). It could be concluded the addition of 5 and 10 µM ergothioneine in the semen extender and thawing temperature at 60˚C in 5 s can be an efficient strategy to preserve rooster cryopreserved semen quality.
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