Evidence map›Paper›PMID 34462831›Full record

ArticleMolecular biology reports2021

Development of the first microsatellite markers using high-throughput sequencing for a hexaploid coastal species, Tournefortia argentea L. f. (Boraginaceae).

Miaomiao Shi, Qiubiao Zeng, Tieyao Tu, Dianxiang Zhang

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Miaomiao ShiKey Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
Qiubiao ZengNational Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, China.
Tieyao TuKey Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
Dianxiang ZhangKey Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China. dx-zhang@scbg.ac.cn.ORCID http://orcid.org/0000-0001-6549-8872
Beijing Botanical Garden · CNGuangxi University · CN

Funding

biological resources program, chinese academy of sciences KFJ-BRP-017-35national natural science foundation of china 32170232
6 · The paper itself

Abstract

backgroudTournefortia argentea L. f. is a hexaploid shrub or tree species with ecological and evolutionary significances, which forms the fringe of vegetation closest to the sea on tropical coral islands. Previous studies have never addressed on genetic information, and thus genomic resources remain scarce. METHODS AND

resultsWe used nine individuals from different islands to identify polymorphic microsatellites of T. argentea by Illumina high-throughput sequencing. Thirty-five polymorphic microsatellite markers were developed. Characteristics of each locus were tested using 48 individuals collected from three populations of T. argentea. A total of 320 alleles were found across the 35 microsatellite loci. The number of alleles per locus ranged from 5 to 15, with an average of 9.1. Observed and expected heterozygosities in each locus per population varied from 0.000 to 1.000 and from 0.000 to 0.893, respectively.

conclusionsIn this study, we report the development of 35 polymorphic microsatellite markers based on Illumina high-throughput sequencing. These markers will facilitate the investigations of genetic diversity, population structures and evolutionary history of T. argentea.

Indexed as

High-Throughput Nucleotide SequencingPolyploidyAquatic OrganismsBoraginaceaeMicrosatellite RepeatsPolymorphism, GeneticPrincipal Component AnalysisBoraginaceaeMicrosatellite markersPolyploidyPopulation genetic structureTournefortia argentea

Identifiers

PMID34462831
OpenAlexW3197146381

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.