ArticleBiotechnology for biofuels2021
Establishment of a resource recycling strategy by optimizing isobutanol production in engineered cyanobacteria using high salinity stress.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 24 citations in OpenAlex.
- Synthetic biology promotes the capture of CO2 to produce fatty acid derivatives in microbial cell factories.Bioresources and bioprocessing · 2022Review
- Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels.Bioengineered · 2022Review
- Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria.Microbial cell factories · 2022Article
- Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria.Biotechnology for biofuels · 2021Review
- Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for COBiotechnology for biofuels · 2021Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIsobutanol is an attractive biofuel with many advantages. Third-generation biorefineries that convert CO
resultsIn this study, we first engineered Synechococcus elongatus for isobutanol biosynthesis by introducing five exogenous enzymes, reaching a production titer of 0.126 g/L at day 20. It was then discovered that high salinity stress could result in a whopping fivefold increase in isobutanol production, with a maximal in-flask titer of 0.637 g/L at day 20. Metabolomics analysis revealed that high salinity stress substantially altered the metabolic profiles of the engineered S. elongatus. A major reason for the enhanced isobutanol production is the acceleration of lipid degradation under high salinity stress, which increases NADH. The NADH then participates in the engineered isobutanol-producing pathway. In addition, increased membrane permeability also contributed to the isobutanol production titer. A cultivation system was subsequently developed by mixing synthetic wastewater with seawater to grow the engineered cyanobacteria, reaching a similar isobutanol production titer as cultivation in the medium.
conclusionsHigh salinity stress on engineered cyanobacteria is a practical and feasible biotechnology to optimize isobutanol production. This biotechnology provides a cost-effective approach to biofuel production, and simultaneously recycles chemical nutrients from wastewater and seawater.
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