ArticleClinical oral investigations2022
Combination of enamel matrix derivative and hyaluronic acid inhibits lipopolysaccharide-induced inflammatory response on human epithelial and bone cells.
Article in Clinical oral investigations, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it, 18 citations in OpenAlex.
- Enamel matrix derivative in the treatment of tooth replantation: from a biological basis to clinical application.Annals of medicine · 2024Pooled it
- Efficiency of Hyaluronic Acid in Infrabony Defects: A Systematic Review of Human Clinical Trials.Medicina (Kaunas, Lithuania) · 2022Pooled it
- Comparision of crosslinked hyaluronic acid vs. enamel matrix derivative for periodontal regeneration: an 18-month follow-up randomized clinical trial.Clinical oral investigations · 2025Trial
- Clinical and Radiographic Evaluation of Intrabony Periodontal Defects Treated with Hyaluronic Acid or Enamel Matrix Proteins: A 6-Month Prospective Study.Oral health & preventive dentistry · 2024Trial
- Prospective effect of mesenchymal exosomes versus nanocurcumin-loaded mesenchymal exosomes on induced periodontitis in albino rats.Odontology · 2026Article
- The Emerging Role of Hyaluronic Acid as a Multifunctional Regenerative Agent in Periodontal Healing.Gels (Basel, Switzerland) · 2026Review
- Enamel Matrix Derivative in Dental Pulp Regeneration: Biological Basis, Mechanistic Insights, and Translational Considerations.Stem cells international · 2026Review
- Multiomics Comparison of Proline-Rich Peptide-Enhanced Hyaluronic Acid Gels Versus Conventional Regenerative Materials: An Early Wound-Healing Model.Journal of periodontal research · 2025Article
- Research progress of enamel matrix derivative on periodontal tissue regeneration: a narrative review.Frontiers in dental medicine · 2025Review
- The role of enamel matrix derivatives in periodontal regeneration following tooth replantation/transplantation: a narrative review of post-reperfusion injury.Frontiers in cell and developmental biology · 2025Review
- Natural Epithelial Barrier Integrity Enhancers-Gels (Basel, Switzerland) · 2024Article
- Lidocaine-Loaded Thermoresponsive Gel for Accelerated Wound Healing in Dry Socket and Oral Wounds.Gels (Basel, Switzerland) · 2024Article
- TGF-β Signalling Mediates the Anti-Inflammatory Activity of Enamel Matrix Derivative In Vitro.International journal of molecular sciences · 2022Article
- Enamel Matrix Derivative Decreases Pyroptosis-Related Genes in Macrophages.International journal of molecular sciences · 2022Article
- Assessment of Changes in the Oral Microbiome That Occur in Dogs with Periodontal Disease.Veterinary sciences · 2021Article
- Hyaluronic acid: Hope or hype in periodontics - A narrative review.Journal of Indian Society of PeriodontologyReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe aim of this study was to evaluate the in vitro effect of enamel matrix derivative (EMD) and hyaluronic acid (HA) and their synergistic combination on lipopolysaccharides (LPS)-induced inflammation in human keratinocytes and osteoblasts. MATERIAL AND
methodsCells were challenged with LPS (1 μg/ml) and cultured in the following treatment groups with EMD (30 mg/ml) and HA (30 mg/ml): LPS, EMD, HA, EMD + HA, EMD + LPS, HA + LPS, and EMD + HA + LPS. Cell viability, inflammatory cytokine expression, and cell migration were determined using colorimetric assay, quantitative real-time polymerase chain reaction (qPCR), and scratch wound healing assay, respectively.
resultsCell viability was decreased when exposed to LPS compared to the controls. Overall, LPS treatment expressed upregulation on inflammatory cytokine tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6). EMD and HA reduced up to 3.0-fold the cytokine expression caused by LPS (p < 0.05). EMD and HA statistically induced higher migration in osteoblasts and keratinocytes, respectively. Migration was impaired by LPS, whereas it significantly increased after addition of EMD and HA.
conclusionsEMD and HA are advantageous biomaterials that individually generate strong directional migratory keratinocyte and osteoblast response. Their combination also enhances cell viability, and anti-inflammatory and migratory abilities to promote healing specially under LPS inflammatory stimulus. Future in vivo and animal research is necessary to further characterize the effect of EMD and HA on periodontal regeneration. CLINICAL RELEVANCE: The use of EMD in conjunction with HA resulted in a reduction of inflammation and improvement of tissue healing at wound sites. Both biomaterials combined may potentially improve the effectiveness of bone regeneration in periodontal bone defects, pointing to the potential clinical relevance of both materials in regenerative periodontal surgery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.