ArticlePlant biotechnology journal2022
Regulation of germination by targeted mutagenesis of grain dormancy genes in barley.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- The SD1 locus affects primary seed dormancy in winter malting barley.The plant genome · 2026Article
- CRISPR/Cas Genome Editing and Its Applications in Cereal Crop Improvement.Plant-environment interactions (Hoboken, N.J.) · 2026Review
- Early Plant Development as a Systems-Level Trait: Integrating Omics, Artificial Intelligence, and Emerging Biotechnologies.Plants (Basel, Switzerland) · 2026Review
- Detection of bsd1, a gene responsible for breaking seed dormancy by machine threshing in barley (Hordeum vulgare L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Translating research on seed dormancy and germination from Arabidopsis to temperate cereals to control pre-harvest sprouting.Frontiers in plant science · 2026Review
- QTL detection, validation, candidate genes and KASP markers for pre-harvest sprouting tolerance in white grained common wheat.BMC plant biology · 2025Article
- Breeding Wheat (Plants (Basel, Switzerland) · 2025Review
- Alanine aminotransferase contributes to hypoxia sensitivity and dormancy in barley seeds.The plant genome · 2025Article
- Rapid development of naked malting barley germplasm through targeted mutagenesis.Molecular breeding : new strategies in plant improvement · 2025Article
- Utilizing CRISPR-based genetic modification for precise control of seed dormancy: progress, obstacles, and potential directions.Molecular biology reports · 2025Review
- Fine mapping of the major-effect QTLFrontiers in plant science · 2025Article
- Role of alanine aminotransferase in crop resilience to climate change: a critical review.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Utilizing Short Interspersed Nuclear Element as a Genetic Marker for Pre-Harvest Sprouting in Wheat.Plants (Basel, Switzerland) · 2024Article
- Identification of Quantitative Trait Loci and Candidate Genes Controlling Seed Dormancy in Eggplant (Genes · 2024Article
- Targeted Modification of Grain Dormancy Genes in Barley.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Malting quality and preharvest sprouting traits are genetically correlated in spring malting barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- QTL Mapping for Wheat Seed Dormancy in a Yangmai16/Zhongmai895 Double Haploid Population.Plants (Basel, Switzerland) · 2023Article
- Transcriptome analysis of knockout mutants of rice seed dormancy gene OsVP1 and Sdr4.Plant cell reports · 2023Article
- Novel resistance to the Bymovirus BaMMV established by targeted mutagenesis of the PDIL5-1 susceptibility gene in barley.Plant biotechnology journal · 2023Article
- Characteristics of the Seed Germination and Seedlings of Six Grape Varieties (Plants (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High humidity during harvest season often causes pre-harvest sprouting in barley (Hordeum vulgare). Prolonged grain dormancy prevents pre-harvest sprouting; however, extended dormancy can interfere with malt production and uniform germination upon sowing. In this study, we used Cas9-induced targeted mutagenesis to create single and double mutants in QTL FOR SEED DORMANCY 1 (Qsd1) and Qsd2 in the same genetic background. We performed germination assays in independent qsd1 and qsd2 single mutants, as well as in two double mutants, which revealed a strong repression of germination in the mutants. These results demonstrated that normal early grain germination requires both Qsd1 and Qsd2 function. However, germination of qsd1 was promoted by treatment with 3% hydrogen peroxide, supporting the notion that the mutants exhibit delayed germination. Likewise, exposure to cold temperatures largely alleviated the block of germination in the single and double mutants. Notably, qsd1 mutants partially suppress the long dormancy phenotype of qsd2, while qsd2 mutant grains failed to germinate in the light, but not in the dark. Consistent with the delay in germination, abscisic acid accumulated in all mutants relative to the wild type, but abscisic acid levels cannot maintain long-term dormancy and only delay germination. Elucidation of mutant allele interactions, such as those shown in this study, are important for fine-tuning traits that will lead to the design of grain dormancy through combinations of mutant alleles. Thus, these mutants will provide the necessary germplasm to study grain dormancy and germination in barley.
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