Evidence map›Paper›PMID 34458834›Full record

ArticleRSC chemical biology2021

A self-labeling protein based on the small ultra-red fluorescent protein, smURFP.

John-Hanson Machado, Richard Ting, John Y Lin, Erik A Rodriguez

Abstract read
In one paragraph

Article in RSC chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025
    Review
  2. Article
  3. Article
  4. Article
  5. Therapeutic Ultrasound for Topical Corneal Delivery of Macromolecules.Translational vision science & technology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

John-Hanson MachadoDepartment of Chemistry, The George Washington University Washington DC 20052 USA erik_rodriguez@gwu.edu.
Richard TingDepartment of Radiology, Molecular Imaging Innovations Institute (MI3), Weill Cornell Medicine New York NY 10065 USA.
John Y LinTasmanian School of Medicine, University of Tasmania Hobart Tasmania 7000 Australia.
Erik A RodriguezDepartment of Chemistry, The George Washington University Washington DC 20052 USA erik_rodriguez@gwu.edu.ORCID https://orcid.org/0000-0001-9143-2535

Funding

Optogenetic mapping of synaptic activity and control of intracellular signalingU01NS090590 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KLEINFELD, DAVID, LIN, JOHN YU-LUEN · 2014 to 2016
$1.2M
NINDS NIH HHS U01 NS090590
6 · The paper itself

Abstract

Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development.

Identifiers

PMID34458834
PMCPMC8341759

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.