Evidence map›Paper›PMID 34458832›Full record

ArticleRSC chemical biology2021

One-pot fluorescent assay for sensitive detection of APOBEC3A activity.

Bingyao Wang, Xiong Zhang, Yafen Wang, Kun Chen, Fang Wang, Xiaocheng Weng, Xiang Zhou

Open access · goldAbstract read
In one paragraph

Article in RSC chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Bingyao WangThe Institute of Advanced Studies, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University Wuhan Hubei 430072 China xzhou@whu.edu.cn xcweng@whu.edu.cn +86-27-68756663 +86-27-68756663.
Xiong ZhangThe Institute of Advanced Studies, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University Wuhan Hubei 430072 China xzhou@whu.edu.cn xcweng@whu.edu.cn +86-27-68756663 +86-27-68756663.
Yafen WangThe Institute of Advanced Studies, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University Wuhan Hubei 430072 China xzhou@whu.edu.cn xcweng@whu.edu.cn +86-27-68756663 +86-27-68756663.
Kun ChenThe Institute of Advanced Studies, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University Wuhan Hubei 430072 China xzhou@whu.edu.cn xcweng@whu.edu.cn +86-27-68756663 +86-27-68756663.ORCID https://orcid.org/0000-0003-0533-8564
Fang WangWuhan University School of Pharmaceutical Sciences Wuhan 430071 China.
Xiaocheng WengThe Institute of Advanced Studies, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University Wuhan Hubei 430072 China xzhou@whu.edu.cn xcweng@whu.edu.cn +86-27-68756663 +86-27-68756663.ORCID https://orcid.org/0000-0002-0605-8495
Xiang ZhouThe Institute of Advanced Studies, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University Wuhan Hubei 430072 China xzhou@whu.edu.cn xcweng@whu.edu.cn +86-27-68756663 +86-27-68756663.ORCID https://orcid.org/0000-0002-1829-9368
Wuhan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.

Identifiers

PMID34458832
PMCPMC8341802
OpenAlexW3162177228

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.