Evidence map›Paper›PMID 34458582›Full record

ArticleOpen life sciences2021

Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms.

Ntelekwane G Khasapane, Jane S Nkhebenyane, Stanford Kwenda, Zamantungwa T H Khumalo, Phillip S Mtshali, Moeti O Taioe, Oriel M M Thekisoe

Open access · goldAbstract read
In one paragraph

Article in Open life sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Ntelekwane G KhasapaneDepartment of Life Sciences, Centre for Applied Food Sustainability and Biotechnology, Central University of Technology, Bloemfontein, 9300, South Africa.
Jane S NkhebenyaneDepartment of Life Sciences, Centre for Applied Food Sustainability and Biotechnology, Central University of Technology, Bloemfontein, 9300, South Africa.
Stanford KwendaSequencing Core Facility, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa.
Zamantungwa T H KhumaloSequencing Core Facility, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa.
Phillip S MtshaliSequencing Core Facility, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa.
Moeti O TaioeEpidemiology, Parasites and Vectors, Agricultural Research Council - Onderstepoort Veterinary Research (ARC-OVR), Onderstepoort 0110, South Africa.
Oriel M M ThekisoeUnit for Environmental Sciences and Management, North-West University, Potchefstroom, 2531, South Africa.
National Health Laboratory Service · ZACentral University of Technology · ZANorth-West University · ZAOnderstepoort Veterinary Institute · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mastitis is a cow disease usually signalized by irritation, swelling, and soreness of the udder. It is characterized by physical, chemical, and biological changes in the udder and milk. The aim of this study was to detect and characterize pathogens causing subclinical mastitis (SCM) from the milk of dairy cows of small-scale farmers through culture and molecular techniques. Milk was collected from 32 cows belonging to 8 small-scale farmers around Harrismith District, South Africa. The results showed that screening of SCM by California mastitis test and somatic cell counts (SCC) was 21.87 and 25%, respectively. Culture methods revealed the presence of

Indexed as

16S ribosomal RNADNA sequencingmastitismicrobial milk compositionmicrobiota

Identifiers

PMID34458582
PMCPMC8374232
OpenAlexW3194738824

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.