ArticleBiomedical optics express2021
Quantitation of cerebral oxygen tension using phasor analysis and phosphorescence lifetime imaging microscopy (PLIM).
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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7 authors at 1 institution in 2 countries.
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Abstract
Time-domain measurements for fluorescence lifetime imaging microscopy (FLIM) and phosphorescence lifetime imaging microscopy (PLIM) are conventionally computed by nonlinear curve fitting techniques to model the time-resolved profiles as mono- or multi-exponential decays. However, these techniques are computationally intensive and prone to fitting errors. The phasor or "polar plot" analysis method has recently gained attention as a simple method to characterize fluorescence lifetime. Here, we adapted the phasor analysis method for absolute quantitation of phosphorescence lifetimes of oxygen-sensitive phosphors and used the phasor-derived lifetime values to quantify oxygen partial pressure (pO2) in cortical microvessels of awake mice. Our results, both experimental and simulated, demonstrate that oxygen measurements obtained from computationally simpler phasor analysis agree well with traditional curve fitting calculations. To our knowledge, the current study constitutes the first application of the technique for characterizing microsecond-length, time-domain phosphorescence measurements and absolute,
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