ArticleBiomedical optics express2021
Quantitative structural imaging of keratoconic corneas using polarization-resolved SHG microscopy.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Reduced corneal toxicity following whole brain irradiation in mice.Clinical and translational radiation oncology · 2026Article
- Characterization of Collagen Fiber Organization in Breast Cancer via Model-Free Multiscale pSHG Image Analysis.Annals of biomedical engineering · 2026Article
- Role of Neural Crest Cells in Establishing Corneal Transparency During Embryonic Development in Mice.Journal of cellular physiology · 2026Article
- Fgfr3-Wnt signaling crosstalk is involved in maintaining cranial suture integrity.Bone research · 2026Article
- Optical characterization of ocular collagen microstructure and anisotropy in animal and human eyes.Biomedical optics express · 2026Article
- Article
- Multiscale investigation of collagen structure in human skin and gel matrices using polarization resolved second harmonic generation microscopy.Scientific reports · 2025Article
- A gene-encoded bioprotein second harmonic generation (SHG) probe from Autographa californica nuclear polyhedrosis virus (AcMNPV) polyhedrin for live cell imaging.European biophysics journal : EBJ · 2025Article
- Quantifying choroidal and retinal thicknesses variations via optical coherence tomography in different stages of pediatric keratoconus.BMC ophthalmology · 2024Article
- Quantitative Assessment of Collagen Remodeling during a Murine Pregnancy.ACS photonics · 2024Article
- Investigating cartilage-related diseases by polarization-resolved second harmonic generation (P-SHG) imaging.APL bioengineering · 2024Article
- On the quantitative analysis of lamellar collagen arrangement with second-harmonic generation imaging.Biomedical optics express · 2024Article
- Impact of corpus callosum fiber tract crossing on polarimetric images of human brain histological sections:Journal of biomedical optics · 2023Article
- Unveiling the lamellar structure of the human cornea over its full thickness using polarization-resolved SHG microscopy.Light, science & applications · 2023Article
- High numerical aperture imaging allows chirality measurement in individual collagen fibrils using polarization second harmonic generation microscopy.Nanophotonics (Berlin, Germany) · 2023Article
- Second harmonic generation microscopy: a powerful tool for bio-imaging.Biophysical reviews · 2023Review
- Two-Photon Imaging for Non-Invasive Corneal Examination.Sensors (Basel, Switzerland) · 2022Review
- Revealing the Wonder of Natural Photonics by Nonlinear Optics.Biomimetics (Basel, Switzerland) · 2022Article
- PSHG-TISS: A collection of polarization-resolved second harmonic generation microscopy images of fixed tissues.Scientific data · 2022Article
- Polarization and Orbital Angular Momentum of Light in Biomedical Applications: feature issue introduction.Biomedical optics express · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human cornea is mainly composed of collagen fibrils aligned together within stacked lamellae. This lamellar structure can be affected in pathologies such as keratoconus, which is characterized by progressive corneal thinning and local steepening. In this study, we use polarization-resolved second harmonic generation (P-SHG) microscopy to characterize 8 control and 6 keratoconic human corneas. Automated processing of P-SHG images of transverse sections provides the collagen orientation in every pixel with sub-micrometer resolution. Series of P-SHG images recorded in the most anterior region of the stroma evidence sutural lamellae inclined at 22° ± 5° to the corneal surface, but show no significant difference between control and keratoconic corneas. In contrast, series of P-SHG images acquired along the full thickness of the stroma show a loss of order in the lamellar structure of keratoconic corneas, in agreement with their defective mechanical properties. This structural difference is analyzed quantitatively by computing the entropy and the orientation index of the collagen orientation distribution and significant differences are obtained along the full thickness of the stroma. This study shows that P-SHG is an effective tool for automatic quantitative analysis of structural defects of human corneas and should be applied to other collagen-rich tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.